Sequences from confirmation amplicon


Confirmation sequences for line 145D01 and hit At5g10140 are shown.
Line ID 145D01
Locus At5g10140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3176443
Primers used for PCR 04gg - 8474, show primer details
   
Sequence Name 43-K111955-0022-145-D01C-04gg
GenBank Accession LN504882 [GenBank]
Sequencing primer gene specific primer, 04gg: 5'-CAAACAAAGAGCTGATGTTACCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K111955-0022-145-D01C-04gg
TGAAGAGGCGTTTTTAAAATTCTTTTTAAACTGGGATACAAAAAGAAAAGAGGTAACTAA
TAATTTGATACCATTGTTCGTAGTCCTGATCAAATGTTATAAGGGTAAACATGATAGAAA
ATAGAGGGTAAATAGGTTTTGTTCTTATAATGGTTTTGATAACACGCTTTGTAAAGGATA
TAGGTGTTTTTTGATGCTAAAAGTTGTGGTATGGATCAAAACCAAAATGGAAGCTCTGAA
TCTCTGATAGAGGTTGCAATTAGAATTATATAAGTTAATTTGCAAATGAATTGGAAGCAG
TCTTCCACTATTTGCTATTGTTAGCAATGAGCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATA
   
Sequence Name 35-K111954-0022-145-D01C-8409
GenBank Accession LN501880 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K111954-0022-145-D01C-8409
ATTTACATTGCTCATTGCTAACATAGCAAATAGTGGAAGACTGCTTCCAATTCATTTGCA
AATTAACTTATATAATTCTAATTGCAACCTCTATCAGAGATTCAGAGCTTCCATTTTGGT
TTTGATCCATACCACAACTTTTAGCATCAAAAAACACCTATATCCTTTACAAAGCGTGTT
ATCAAAACCATTATAAGAACAAAACCTATTTACCCTCTATTTTCTATCATGTTTACCCTT
ATAACATTTGATCAGGACTACGAACAATGGTATCAAATTATTAGTTACCTCTTTTCTTTT
TGTATCCCAGTTTTAAAAAGAATTTTTTAAAAACGCCTCTTTCATGAGTTCATTAGTAAT
CTCGTGTCAAAATTGGTAACATCAGCTCTTTGTTTG

Last Updated on 06.12.2018 16:11