Sequences from confirmation amplicon


Confirmation sequences for line 145D08 and hit At5g10540 are shown.
Line ID 145D08
Locus At5g10540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3330774
Primers used for PCR 12kh - 8474, show primer details
   
Sequence Name 75-K113756-0022-145-D08C-12kh
GenBank Accession LN513279 [GenBank]
Sequencing primer gene specific primer, 12kh: 5'-TTCATGGAACACAGTCTCTACCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K113756-0022-145-D08C-12kh
TGAGTCATTGACCATGCATGTAAGAGTTCTCCAAAAGCACCTACATTTTCAAGTATGGAA
AGCTACATATAGGTCGAAAAGAGAAGTGGTCTATCTAGTGCTACTCCTAAAGATTGTAAA
ATTTCGTCATAGCATGGCCCTCCACGCCACCAATATGTAAAGTTCCATGAGTGAATACCA
ATCCACAATAGTCGAATAAATCAATAAAATTACACATGAAGTAAGTTTTCTCAGGTACCA
ATTTAACTTCTAGGGCAGGGAAAACTACATTTGGGGGCATGTGTACTCATAGTTTGTTAT
AATAGGGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCACGGTTATTATA
   
Sequence Name 37-K113755-0022-145-D08C-8409
GenBank Accession LN502686 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K113755-0022-145-D08C-8409
ATTTACAATTGAATAATCCTGCCCTATTATAACAAACTATGAGTACACACGCTCCCAAAT
GTAGTTTTCCCTGCCCTAGAAGTTAAATTGGTACCTGAGAAAACTTACTTCATGTGTAAT
TTTATTGATTTATTCGACTATTGTGGATTGGTATTCACTCATGGAACTTTACATATTGGT
GGCGTGGAGGGCCATGCTATGACGAAATTTTACAATCTTTAGGAGTAGCACTAGATAGAC
CACTTCTCTTTTCGACCTATATGTAGCTTTCCATACTTGAAACTTTAGGTGCTTTTGGAG
AACTCTTACATGCATGGTCAATGACTCATGATCCTTGTTCTTTATGACAATATTATTCTG
ATTTACAGGTAGAGACTGTGNTCCATGAAA

Last Updated on 06.12.2018 16:11