Sequences from confirmation amplicon


Confirmation sequences for line 145E12 and hit At5g48570 are shown.
Line ID 145E12
Locus At5g48570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19690928
Primers used for PCR BS03 - 8474, show primer details
   
Sequence Name 10-K020068-022-145-E12C-8409
GenBank Accession LN490448 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K020068-022-145-E12C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCTGCATTTACAAAGCC
ATTTACATGTAAAACTTAGCATCTTTCTTGTTATACTCTTTCACCTTCTCCTTCAACTTC
TTATATTCTATCTTCACCTCCCTGTGCATACCCATGTTGGAAATTAGAATACATCGCAAC
GAAAAAAACAAAGGACCAATAGAAAATTACTCACTTGTTGTCTGGATCGATTTCGAGAGC
TTTCTTGATATCAAGCTCAGCCAGATCAAGATCCGCCGTCTCCAAGTAGGCATGTGCTCT
CCTATACATTGCCTTCACGTTCCTACTATCCATCTCCAACACCTATTCACATTCGTTTCA
TTCACTACTTAGTCGTTCATCTCCAATTGTAATATCCAAAATTACTGAAACTGAAATGTT
TTGTTTTTACCTTTGTGGACAATTTTGCTGCTTCCTTGTAATCTTTCAGTTTCAGCTTGC
AAGCTGCATCATTCAGGTTACGGCAATCTGAGATCTTTTGATTTCTTTTTCTCCTCTTCG
TCGAAAGTTGAGTCATATTCTATATATTTGACACCCTGCAATCAAAACTTGATGTTAAAA
TCTAAACAAAAACATAAGTTTCTTTAGCAAATAC
   
Sequence Name 09-K020068-022-145-E12C-BS03
GenBank Accession LN489976 [GenBank]
Sequencing primer gene specific primer, BS03: 5'-TTTGGGATTTGGTCCTAGTATTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K020068-022-145-E12C-BS03
AGAAACTTATGTTTTTGTTTAGATTTTAACATCAAGTTTTGATTGCAGGGTGTCAAATAT
ATAGAATATGACTCAACTTTCGACGAAGAGGAGAAAAAGAAATCAAAAGATCTCAAGATT
GCCTGTAACCTGAATGATGCAGCTTGCAAGCTGAAACTGAAAGATTACAAGGAAGCAGCA
AAATTGTCCACAAAGGTAAAAACAAAACATTTCAGTTTCAGTAATTTTGGATATTACAAT
TGGAGATGAACGACTAAGTAGTGAATGAAACGAATGTGAATAGGTGTTGGAGATGGATAG
TAGGAACGTGAAGGCAATGTATAGGAAAGCACATGCCTACTTGGAGACGGCGGATCTTGA
TCTGGCTGAGCTTGATATCAAAAAAGCTCTCGAAATCGATCCAGACAACAAGTGAGTAAT
TTTCTATTGGTCCTTTGTTTTTTTCGTTGCGATGTATTCTAATTTCCAACATGGGTATGC
ACAGGGAGGTGAAGATAGAATA

Last Updated on 06.12.2018 16:11