Genotyping and primer details


Genotyping and primer details for 145E12 - At5g48570
Line ID 145E12
Locus At5g48570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19690928 of line 145E12 Chr5:19690928
   
Insertion allele amplicon 8474 - BS03
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BS03: 5'-TTTGGGATTTGGTCCTAGTATTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19691513
   
wt amplicon (over InsPos) 34hs - BS03
wt amplicon start Chr5:19690330
wt amplicon end Chr5:19691513
wt amplicon size 1184 bp
Locus specific primer BS03: 5'-TTTGGGATTTGGTCCTAGTATTTG-3'
2nd locus specific primer 34hs: 5'-TAATTCCATCACAAAAGGTTTTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11