Sequences from confirmation amplicon


Confirmation sequences for line 146D10 and hit At4g24860 are shown.
Line ID 146D10
Locus At4g24860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:12803103
Primers used for PCR 19dh - 3144, show primer details
   
Sequence Name 63-K109780-0022-146-D10C-19dh
GenBank Accession LN511009 [GenBank]
Sequencing primer gene specific primer, 19dh: 5'-AAAGATAACGCTAGGGGACATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K109780-0022-146-D10C-19dh
CTTTGACTCTTCTCCCCTCACCAAACCACTGCAGTATTAAGAAGAACACAAGTTTGACAA
AAAGATCCCGTGTGTAAATCGGTAGAACTTTTCATAGAGTATTCAGTTACCTTAGATGTG
ATGCTTGACATTGAGATGTTGATGAAGTTCGCATCAGCTTCCTTTGCCACTGCTTTTGCA
AGCATGGTCTTTCCAGTTCCAGGAGGTCCAAATAAAAGAATTCCTTTACAAGGCTGCATA
AAGACGAACAAGAGCGGACTGATTAAGAAGCCACCAATGCATAACGTTAACTACCAAATT
TTAGGAGAGAAGAATTATTGAGGAAAGCCAAATTAGCAGCTAGTCTCGCAAATAAAAGGT
ATTTCCTTCATACATCTGTGATAAATCCGAGGAAGGATAGTGGGATTGTGCGTCATCCCT
TACGTCAGTGGAGA
   
Sequence Name 47-K109779-0022-146-D10C-3144
GenBank Accession LN506221 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K109779-0022-146-D10C-3144
TCCACTATCCTTCCTCGGATTATCACAGATGTATGAAGGAAATACCTTTTATTTGCCAGA
CTAGCTGCTAATTTGGCTTTCCTCAATAATTCTTCTCTCCTAAAATTTGGTAGTTAACGT
TATGCATTGGTGGCTTCTTAATCAGTCCGCTCTTGTTCGTCTTTATGCAGCCTTGTAAAG
GAATTCTTTTATTTGGACCTCCTGGAACTGGAAAGACCATGCTTGCAAAAGCAGTGGCAA
AGGAAGCTGATGCGAACTTCATCAACATCTCAATGTCAAGCATCACATCTAAGGTAACTG
AATACTCTATGAAAAGTTCTACCGATTTACACACGGGATCTTTTTTTCAAACTTGTGTTC
TTCTTAATACTGCAGTGGTTTGGTGAGGGTGAGAAGTATGTCAAAGCTGTCTTTTCTTTG
GCC

Last Updated on 06.12.2018 16:11