Sequences from confirmation amplicon


Confirmation sequences for line 146D12 and hit At5g18120 are shown.
Line ID 146D12
Locus At5g18120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5993159
Primers used for PCR 05fm - 8474, show primer details
   
Sequence Name 63-K111507-0022-146-D12C-05fm
GenBank Accession LN511016 [GenBank]
Sequencing primer gene specific primer, 05fm: 5'-CTTTCTTGGAAGTTCCTTGTTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K111507-0022-146-D12C-05fm
TCGATCATGTGTAGGGCACGGCCAAACAGTTGGCTAGTCTCACCAAGTATTCCTAAGCTC
AGATGCGGAACATACAATGCCCATAACGTTTTCAACCGTGATCCCATGATGGGGAAGATC
AAAATTGCCAGTTTCAAAGACAGAAACATTAGTGCAAGTACCATATATGGCTCTCTTTCT
GCTATTTCTCTGATAGATGACCCGTTGTGTAGCCATGTGATCAGGTTTCCATCAGTGTCC
AAGCTTGTTGGTTCACCTTCATCCATATACTGGACAGGTTTAAGACCTGGAAAGACACAG
ATATAAAAAAGTTTAACTGATTTTCTTCAAAGGATTTTAATAAGTTGTGAGCTAATATAT
TCAGATACAGTAGAAAACAAAACCAGAAAGTACAAACAGAGACACAGTCTGGCTTTTCAT
GAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCA
   
Sequence Name 51-K111506-0022-146-D12C-8409
GenBank Accession LN507541 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K111506-0022-146-D12C-8409
ATTCATGAAAAGCCAGACTGTGTCTCTGTTTGTACTTCCTTTGTTTTGTTTTCTACTGTA
TCTGAATATATTAGCTCACAACTTATTAAAATCCTTTGAAGAAAATCAGTTAAACTTTTT
TATATCTGTGTCTTTCCAGGTCTTAAACCTGTCCAGTATATGGATGAAGGTGAACCAACA
AGCTTGGACACTGATGGAAACCTGATCACATGGCTACACAACGGGTCATCTATCAGAGAA
ATAGCAGAAAGAGAGCCATATATGGTACTTGCACTAATGTTTCTGTCTTTGAAACTGGCA
ATTTTGATCTTCCCCATCATGGGATCACGGTTGAAAACGTTATGGGCATTGTATGTTCCG
CATCTGAGCTTGGGAATACTTGGTGAGACTAGCCAACTGTTTGGCCGTGCCCTACACATG
ATCGATGTGAGGAGACTATGGATAAAACTGAGACTCACCAAAACAAGGAACTTCCAAGAA
A

Last Updated on 06.12.2018 16:11