Sequences from confirmation amplicon


Confirmation sequences for line 146E11 and hit At4g30940 are shown.
Line ID 146E11
Locus At4g30940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15055993
Primers used for PCR KR60 - 8474, show primer details
   
Sequence Name 95-K100855-0022-146-E11C-8409
GenBank Accession LN515336 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K100855-0022-146-E11C-8409
ACTGTTATTTAGCTTGTTGGATTTCCGAGAGAAGAACATGGTCTGGTCTTGGTCTGATAT
CGGATTTCTTACAATGGCGGAAGAGAAGAGAGTGAGAGACGCGATAGCAATGGAAGAGAG
CAATTCGATATGTGTGGTGAATGAGTTTGAAGATCTAGGGTTTATTGATCTGAGGATGGA
TGGAGGAGGGAGTAGTGTGAGGTGG
   
Sequence Name 95-K100861-0022-146-E11C-KR60
GenBank Accession LN515337 [GenBank]
Sequencing primer gene specific primer, KR60: 5'-CACATCAAACACATTCTCCTCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>95-K100861-0022-146-E11C-KR60
TAGAGAATCACAAATGGAGCCGCCATAGCTTCTTTTCAACCTAGAAGTCAAAACCCAGTC
TGATCCACAAAACACAGAGATGGAATCATTCATAGACGAGAAGAGCTGGCCTTCATGCAA
AGCAAGTTTGGGGTAACAAGGCTCATCAGGCATCTTGCTTTTCATCAACCTGCTCCTAGA
ACTCCACCTCACACTACTCCCTCCTCCATCCATCCTCAGATCAATAAACCCTAGATCTTC
AAACTCATTCACCACACATATCGAATTGCTCTCTTCCATTGCTATCGCGTCTCTCACTCT
CTTCTCTTCCGCCATTGTAAGAAATCCGATATCAGACCAAGACCAGACCATGTTCTTCTC
TCGGAAATCCAACAAGCTAATATAACAGTTATCTTTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11