Sequences from confirmation amplicon


Confirmation sequences for line 146F03 and hit At2g01260 are shown.
Line ID 146F03
Locus At2g01260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:136708
Primers used for PCR 10vd - 3144, show primer details
   
Sequence Name 58-K117751-0022-146-F03C-10vd
GenBank Accession LN509819 [GenBank]
Sequencing primer gene specific primer, 10vd: 5'-AACAGACTCAAACAAGTTCCCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K117751-0022-146-F03C-10vd
TTGACAAAACAAATAGAAAAGAATGTGGTTTAGTTTAAGTTGACGATGACCAGTCCCTGA
GCAGATTGAACATTTCTGATTAGTTGATAGCCATCAGTTGAAGGTTAGAAACAACAAAGG
CCATTTCTGAGCATTAGTTTTTAAATTTGAAGTTACCTGGTATGATATAAGAGCATAAAA
GATGATAATAATATATATCATCATCTTAGTATATAGTATCTACACCATGCTTTCGGTTCT
AGTAGAGAAATGAACGCACAAACCTTATCAGCAAAAGGTTCACGGATGTATGGAAGGTCT
CTTTCAAGATACTCAAACATCAAACGACCTTGAGAGCCTAAAGGTTCGCAATACAGGTAT
TTTGGATTAGTATGATGTTAAACGACCTTAAACACTGATAGTTTAAACCGAAGGCGGGAA
ACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAG
AGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATA
   
Sequence Name 33-K117750-0022-146-F03C-35St
GenBank Accession LN501168 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K117750-0022-146-F03C-35St
TTCGCAGACCCTTCCTCATATAAGGAAGTTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTAAGGTCGTTTAAC
ATCATACTAATCCAAAATACCTGTATTGCGAACCTTTAGGCTCTCAAGGTCGTTTGATGT
TTGAGTATCTTGAAAGAGACCTTCCATACATCCGTGAACCTTTTGCTGATAAGGTTTGTG
CGTTCATTTCTCTACTAGAACCGAAAGCATGGTGTAGATACTATATACTAAGATGATGAT
ATATATTATTATCATCTTTTATGCTCTTATATCATACCAGGTAACTTCAAATTTAAAAAC
TAATGCTCAGAAATGGCCTTTGTTGTTTCTAACCTTCAACTGATGGCTATCAACTAATCA
GAAATGTTCAATCTGCTCAGGGACTGGTCATCGTCAACTTAAACTAAACCACATTCTTTT
CTATTTGTTTTAGTCAACAAACTAGACCACATTCTAACTCACAAAAAAATTGGGAACTT

Last Updated on 06.12.2018 16:11