SimpleSearch - Line and FST details
Line specific information
| Line ID | 146F03 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N413983) |
| Segregation Analysis | 50:22:13 |
| Confirmed for Hit | At2g01260 At2g01260 |
| Parent of DUPLO pair | 12809 |
| Parent of pair(s) | none |
| Note | |
Gene hit At2g01260
| Sequence (A. th genome BLAST matches underlined) | >22-K013035-022-146-F03-35St AATCATTGACGTAAGGGACGACATCTATTCCCACTATCCTTACCAAGACCCTTCCTCTAT ATAAGGAATGTCATTTCATTTGGAGAGGACAGGGCTACCCGGGGATCAGATTGTCGTTTC CCGCCTTCGGTTTAAACTATCAGTGTTTAAGGTCGTTTAACATTCATACTAATCCAAAAT ACCTGTATTGCGAACCTTTATGCTCTCAAGGTCGTTTGATGTTAGAGTATCTTGAAAGAG ACCTTCCATACATCCGTGAACCTTTTGCTGATAAGGTTTGTGCGTTCATTTCTCTACTAT GGGATCCTCCCTATAGTGGCGNTTTACCCTTAAAGGCCGAAGGCATAAACAAATTATTCT AATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGGGGGCTTAGATGAA AAACTTCACGATCGATGCCTTGATTTCGCCATTCCCAGATACCCATTTCATCTTCAGATT GGTCTGAGATTATGCGAAAATATACACTCATATACATAAATACTGACAGTTTGAGCTACC AATTCAGTGTAGCCCATTACCTTACATAATTCACTCAAATGCTATGGGATCCTCCCTATA GTGA |
| GenBank Accession | FR804371 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr2:136742 - go to primer design |
| BLAST e Value | 1e-50 |
| Hit Clone Code (BAC ID) | F10A8 |
| Hit Gene Code | At2g01260 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | hypothetical protein (DUF789) |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At2g01260
| Sequence (A. th genome BLAST matches underlined) | >22-K104555-0022-146-F03-8409 CGGACATGAGCATTTACAATTGAATATATCCTGTAAAATACCTTTAGGAATGTTTCAAGA TACTTATGTAGATGGTGCATAGGAATGATTTGGGGTTATGAATGTAACATTTTAAAATCT GATGAGAAATATGTGACTATGGGAAATATAAAGGGGTTAAACATTGGCAAAAGCCAAAGG CCATATGTATATTAGGAGGCTCTTTAATTTAATGGTTGATACTCGATGCTTGCTTGCTAT GGGATCCTCCCTATAGTGAGACCTATTGGACCCTCCCTATACTGGGCTACATTGAATTCT GAGCTCAATCCATTGCATCNTTATTTTAGTAGGANGAAAAAGGTCAAAAAACAAAAACTA TGACAAGAATGAAAAGAGAGAAGGTGAGAATTATCTNATACAGAGCACACTGAAAAGACA GCGAATNGAGCAATAGTCCAGAGAGGGGAGCAGAGGGGGGGAGCAAAAATAGGTGCCAAC GATGGCGAGCCCATTGGAAGGCAGGGGAAAGAGAGGATTTCGGAAGAAAAGAAGGGCGGA GAAAGACGCAGAAAAACTGAAAGGAGGAGAGAAAGGGGGGGAAAGGGGATAGAAAATGGA GGAGNACAAAGAAGGGCAGAAGAGCAGAGCGAGATAGAGTGCGGGAAGAAAGACGGAGTA GCAATTAATAAGACTACATGGTGCAAGAGAGACGAAGAAGAATGAGAAAGTAGAGGGAAC TAAGGTAACAGAGAGGAATGAGGGAAAAATGAGACAAGAAAGCGGAGCAGAGGAAAACAG CGAGAAAGAGATAAAGTGACAGAC |
| GenBank Accession | FR804372 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr2:137999 - go to primer design |
| BLAST e Value | 2e-93 |
| Hit Clone Code (BAC ID) | F10A8 |
| Hit Gene Code | At2g01260 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | hypothetical protein (DUF789) |
| Insertion Classification | Promoter |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit At3g01650
| Sequence (A. th genome BLAST matches underlined) | >22-K012980-022-146-F03-8409 NTGATCCATTGTAGATTTCCCGNGACATGAAGCCTTTACAATTGAATATATCCTGCTTAA TACCTTGTAGGAATGTGAACGATACTTATGTAGATGGTGCACTACGAATGATTTGGGGTA TTGAATGTAACATTCTAAAATCTGACTGAGAACACTATAGTGATCTATGGCGTATACACT TATACGGGTTACAACATTGGCAAAAGCCAGACGCCATCTGTTTATTAAGAGTGCTCTTTA ATCTCAATGGGTGATACTCTATGCTTCGCTTGCTATGGGATCCTCCCTATAGTAGTCCTT CTAACTCANNCGGGNTGCTCAGACATATCGGACATAGCTGTTCAAGGGGAAAATAACACA AACGAGTTTAACAACAAAAAGTTCCAAAGCTCTTATTTCACTTAACATTTCAAACAGATT CCTAAGTTTCTACTAATTACCTGAATATCGTCTGCACTACTCATTCGCTTGCTCTCAAAT GGATAACGAGGTACACTCGGTTCAAAATTCGGTATCCGGGAACTATGGGATCCTCCCTAT AGTGAGTCGTATAACT |
| GenBank Accession | AL766064 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:245203 - go to primer design |
| BLAST e Value | 1e-111 |
| Hit Clone Code (BAC ID) | F4P13 |
| Hit Gene Code | At3g01650 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | RING domain ligase1 |
| Insertion Classification | TS2TE (3') |
| Confirmation Status | failed |
Gene hit At3g09090
| Sequence (A. th genome BLAST matches underlined) | >22-K104557-0022-146-F03-8409 GNAGCCATTTACAATTGAATATATCCTGCTCATCACCTTGAGGGTATGTGACACGACACT TACGTAGATAGGGCGCATGAGATGATTAGGGGTATCGATAGTTACCTTCGATCTCCATCT CAGCCCAAAATTTTTTGCCTTCCTCTTCAGGGGTACCTCAGGGTCCAATGCCTGACAGAC ACGCCTTCACTATCATGCCCATTGGCCTTATTGTTCCTGCCGTGACTCGATGGGATCCTC CCCCTCCTGAGAGTGAGAACACC |
| GenBank Accession | FR804373 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr3:2783268 - go to primer design |
| BLAST e Value | 4e-14 |
| Hit Clone Code (BAC ID) | MZB10 |
| Hit Gene Code | At3g09090 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | defective in exine formation protein (DEX1) |
| Insertion Classification | CDSi |
| Confirmation Status | failed |
|
Last Updated on 10.06.2021 13:37 |
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