Sequences from confirmation amplicon


Confirmation sequences for line 146G09 and hit At4g18960 are shown.
Line ID 146G09
Locus At4g18960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10385811
Primers used for PCR MR46 - 3144, show primer details
   
Sequence Name 52-K016293-022-146-G09C-3144
GenBank Accession LN507660 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K016293-022-146-G09C-3144
GGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATT
TCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAA
CTATCAGTGTTTTCCCGGATTTGTTGTCTTTGTTAAAGAATCTTTGATCACGTCATCACT
CAGATATTATTTCTTTTTATTTTTCACTTGTATTTTTACTAATTTATTATAAAGTTTTGT
TAGTTTCAGTTCTTGACTTCTGACAAGAAGGTTTTATGTCATAATGAATTAATTTGTAAC
CTATTTATAAATTCAAAAATGTCATCATATTACTACTTTTGACCATTTAATATTAGATTT
CTCATTTGGTCAATACCCAA
   
Sequence Name 53-K016293-022-146-G09C-MR46
GenBank Accession LN507981 [GenBank]
Sequencing primer gene specific primer, MR46: 5'-ATTGGGTATTGACCAAATGAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K016293-022-146-G09C-mr46
ATGGTCAAAAGTAGTAATATGATGACATTTTTGAATTTATAAATAGGTTACAAATTAATT
CATTATGACATAAAACCTTCTTGTCAGAAGTCAAGAACTGAAACTAACAAAACTTTATAA
TAAATTAGTAAAAATACAAGTGAAAAATAAAAAGAAATAATATCTGAGTGATGACGTGAT
CAAAGATTCTTTAACAAAGACAACAAATCCGGGAAAACACTGATAGTTTAAACCGAAGGC
GGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTA
TATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGA
GATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAACGTCTTCTTTTTCCACGAT
GCTCCTCGTGGGTGGGGGTC

Last Updated on 06.12.2018 16:11