Genotyping and primer details


Genotyping and primer details for 146G09 - At4g18960
Line ID 146G09
Locus At4g18960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10385811 of line 146G09 Chr4:10385811
   
Insertion allele amplicon 3144 - MR46
T-DNA primer 3144: 5'-GTGGATTGATGTGATATCTCC-3'
Locus specific primer MR46: 5'-ATTGGGTATTGACCAAATGAGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10386054
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11