Sequences from confirmation amplicon


Confirmation sequences for line 146G11 and hit At2g40116 are shown.
Line ID 146G11
Locus At2g40116 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16753168
Primers used for PCR KR59 - 3144, show primer details
   
Sequence Name 69-K100822-0022-146-G11C-3144
GenBank Accession LN512146 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K100822-0022-146-G11C-3144
ATCCACTTCCTTCGCAGACCCTTCCTCTAATAAAATCATACAAACGATTCACGCTGGGAA
ACCAAAGGGAACGGTGAAAGAAGAGATGAAAGTTGTGGTTGATAAAGTGAGACGTTTGAG
TTTAAGCGAGCAGGAACTTGACAGGACTTGTTCATCTAATAGTCAGGATGTTGTAAGGTA
GTACTAATTTATATACCATCCTAACTCTTTATAGGCCAGGGTTTGCAACTTGATTAATCT
CAATGTTTTTTTAGGTTTACGCAGCGGAATTTGCTTCGGATATACCCTAAAGGGACAAGA
TTTAACTCCTCAAACTACAAACCTCTTATCGGTTGGACTCACGGAGCGCAAATGATTGCT
TTCAACATGCAGGTAACAAGCTAAGTTTTATAAGGTCTATGGATACCTTGACTCTTAAGC
TACCTTTTGTGATGTATAAAGTGATCTCTGTATAAGAAATGAAACATTTGGTGATTATAG
GGATATGGGAAA
   
Sequence Name 69-K100834-0022-146-G11C-KR59
GenBank Accession LN512148 [GenBank]
Sequencing primer gene specific primer, KR59: 5'-TTTCCCATATCCCTATAATCACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K100834-0022-146-G11C-KR59
CTTTATACATCACAAAAGGTAGCTTAAGAGTCAAGGTATCCATAGACCTTATAAAACTTA
GCTTGTTACCTGCATGTTGAAAGCAATCATTTGCGCTCCGTGAGTCCAACCGATAAGAGG
TTTGTAGTTTGAGGAGTTAAATCTTGTCCCTTTAGGGTATATCCGAAGCAAATTCCGCTG
CGTAAACCTAAAAAAACATTGAGATTAATCAAGTTGCAAACCCTGGCCTATAAAGAGTTA
GGATGGTATATAAATTAGTACTACCTTACAACATCCTGACTATTAGATGAACAAGTCCTG
TCAAGTTCCTGCTCGCTTAAACTCAAACGTCTCACTTTATCAACCACAACTTTCATCTCT
TCTTTCACCGTTCCCTTTGGTTTCCCAGCATGAATCGTTTGTATGATTTGTATTAGAGGA
AGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCA

Last Updated on 06.12.2018 16:11