Sequences from confirmation amplicon


Confirmation sequences for line 146H11 and hit At1g26790 are shown.
Line ID 146H11
Locus At1g26790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9275180
Primers used for PCR IW05 - 8474, show primer details
   
Sequence Name 35-K015842-022-146-H11C-8409
GenBank Accession LN501617 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K015842-022-146-H11C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGGTAATCTTGTCTCAGACCTCAAATAAA
GAATAAACACATAACACAAATCTAATCTCATACTCCATCTTCTTTTCTGTCTTATGTCTT
CAAAGATTGGTATGTCTCAAGTTAGAGATACTCCGGTTAAATTGTTTGGCTGGACAATTA
CACCGGTTTCTCATGATCCATACTCTTCTTCGTCCCATGTTCTTCCTGATTCTTCCTCGT
CTTCCTCGTCTTCTTCTCTATCACTTCGACCACACATGGTATGTTTCATGGTCTTACCTT
TATTTGTTAAGAAATGCTAAAGATTTAATATTTTCTCTAGTCCTTGTATCAGTTTGAAAC
TAGTTCATGATGTTAATCCTAATCCAAGTTCCTTCAATTTTTTTCAGATGAATAACCAAT
CTGTTACTGACAATACAAGTCTTAAGCTGTCATCTAATCTTAACAACGAGTCAAAAGAAA
CATCTGAGAACAGTGATGACCAACACAGCGAGATCACAACAATTACATCGGAAGAAGAGA
AAACAACTGAACTGAAGAAACCAGACAAGATTCTTCCATGTCCGAGATGCAACAGCGCAG
ACACCAAATTCTGTTACTACAACAACTACAACGTTAACCAGCCACGTCACTTCTGTAGAA
AATGCCAGAGGTATTGGACCGCTGGTGGATCCAT
   
Sequence Name 36-K015842-022-146-H11C-IW05
GenBank Accession LN502024 [GenBank]
Sequencing primer gene specific primer, IW05: 5'-GAAACAGGTTCAGCGTTTATCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K015842-022-146-H11C-iw05
GTTTGATTGATGCTTAGGCCTCTCAGTTACCAAAGAGTCCGAAGACTCGAAGCTTAGAAT
CTTTGTTGAGGAGCTATTGTAATTGTCAGTATCCTCGGAAGTGATGTGCAGGTACTGGTC
TGAAGAAACCCATCCCTTGTTCTTGCGACGGCCTGAGCCAACCGGGACGATCCTCATGGA
TCCACCAGCGGTCCAATACCTCTGGCATTTTCTACAGAAGTGACGTGGCTGGTTAACGTT
GTAGTTGTTGTAGTAACAGAATTTGGTGTCTGCGCTGTTGCATCTCGGACATGGAAGAAT
CTTGTCTGGTTTCTTCAGTTCAGTTGTTTTCTCTTCTTCCGATGTAATTGTTGTGATCTC
GCTGTGTTGGTCATCACTGTTCTCAGATGTTTCTTTTGACTCGTTGTTAAGATTAGATGA
CAGCTTAAGACTTGTATTGTCAGTAACAGATTGGTTATTCATCTGAAAAAAATTGAAGGA
ACTTGGATTAGGATTAACATCATGAACTAGTTTCAAACTGATACAAGGACTAGAGAAAAT
ATTAAATCTTTAGCATTTCTTAACAAATAAAGGTAAGACCATGAAACATACCATGTGTGG
TCGAAGTGATAGAGAAGAAGACGAGGAAGACGAGG

Last Updated on 06.12.2018 16:11