Sequences from confirmation amplicon


Confirmation sequences for line 147G08 and hit At1g12290 are shown.
Line ID 147G08
Locus At1g12290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4180264
Primers used for PCR 01rx - 3144, show primer details
   
Sequence Name 33-K116218-0022-147-G08C-01rx
GenBank Accession LN501136 [GenBank]
Sequencing primer gene specific primer, 01rx: 5'-AGATACAAAAAGAGATTGGCGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K116218-0022-147-G08C-01rx
GTGAAACCAAAAGGCAGTCGATATACTCAATTTTCTAAGCAAAAAGAGATTTGTGTTGCT
TTTGGATGACATATGGAAGAGGGTGGAACTAACTGAGATTGGAATCCCGAATCCAACAAG
TGAAAACGGATGCAAAATAGCATTCACCACTCGCTGTCAAAGTGTTTGTGCGAGCATGGG
AGTTCATGATCCGATGGAAGTCCGGTGTTTAGGTGCAGATGATGCGTGGGATTTGTTCAA
GAAAAAAGTTGGAGATATCCAATCAATCCACA
   
Sequence Name 09-K116217-0022-147-G08C-3144
GenBank Accession LN490343 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K116217-0022-147-G08C-3144
CCGGACTTCCATCGGATCATGAACTCCCATGCTCGCACAAACACTTTGACAGCGAGTGGT
GAATGCTATTTTGCATCCGTTTTCACTTGTTGGATTCGGGATTCCAATCTCAGTTAGTTC
CACCCTCTTCCATATGTCATCCAAAAGCAACACAAATCTCTTTTTGCTTAGAAAATTGAG
TATATCGACTGCCTTTTGGTTTTCACTTTTCTGGTTCCATTCCACGCCTATAAATCCTAT
TTTTTCGCCAATCTTTATTTATATCTAAA

Last Updated on 06.12.2018 16:11