Sequences from confirmation amplicon


Confirmation sequences for line 147G08 and hit At4g11610 are shown.
Line ID 147G08
Locus At4g11610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7014609
Primers used for PCR 31wl - 8474, show primer details
   
Sequence Name 36-K118410-0022-147-G08C-31wl
GenBank Accession LN502402 [GenBank]
Sequencing primer gene specific primer, 31wl: 5'-GAGTTTGTCTTGAAGGAGGATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K118410-0022-147-G08C-31wl
CGATCTCGCCCATCCGCTAGACCACTCTGGAGACACCGATCGGAGTTCTTGAGCTCGGGA
TCTTAAACGCTGTTGGACTTCACCCAATGAAAACTAGAGAAGGCAGAGGAACCTCTGACA
CTTTTTGCGTTGGGAAATATGGACAAAAGTGGGTTAGAACAAGGACAATGGTGGATAACT
TGTGTCCCAAGTATAACGAGCAGTATACATGGGAAGTCTTTGACCCAGCGACGGTTTTAA
CCGTTGGTGTGTTTGATAACGGACAGCTAGGTGAAAAAGGAAATAGAGATGTGAAGATTG
GGAAGATTCGTATAAGGTTATCTACTTTAGAGACTGGACGGATCTACACACATTCTTACC
CGCTACTTGTTCTTCACCCTACTGGGGTGAAGAAAATGGGCGAGCTACATATGGCCGTGA
GGTTCACAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCG
   
Sequence Name 26-K118409-0022-147-G08C-8409
GenBank Accession LN498131 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K118409-0022-147-G08C-8409
ATTTACAATTGTGAACCTCACGGCCATATGTAGCTCGCCCATTTTCTTCACCCCAGTAGG
GTGAAGAACAAGTAGCGGGTAAGAATGTGTGTAGATCCGTCCAGTCTCTAAAGTAGATAA
CCTTATACGAATCTTCCCAATCTTCACATCTCTATTTCCTTTTTCACCTAGCTGTCCGTT
ATCAAACACACCAACGGTTAAAACCGTCGCTGGGTCAAAGACTTCCCATGTATACTGCTC
GTTATACTTGGGACACAAGTTATCCACCATTGTCCTTGTTCTAACCCACTTTTGTCCATA
TTTCCCAACGCAAAAAGTGTCAGAGGTTCCTCTGCCTTCTCTAGTTTTCATTGGGTGAAG
TCCAACAGCGTTTAAGATCCCGAGCTCAAGAACTCCGATCGGTTGTCTCCAGAGTGGTCT
AGCGGATGGGCGGAGATCGCTGCTGTAATGAGTTGACTCATCCAAAACATGGTATCCTCC
TTCAGAACAAACTC
Primers used for PCR 68wv - 3144, show primer details
   
Sequence Name 71-K118684-0022-147-G08C-68wv
GenBank Accession LN512637 [GenBank]
Sequencing primer gene specific primer, 68wv: 5'-TTTGGACTCACTGATCCTTTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K118684-0022-147-G08C-68wv
ATGAATACATCCAAAATATTATAACGTTTTCAAATGAAAAAAAAATTGTAGATAAAACAA
ATCCAGATTACAAATAGTAACATAAGTCTATATCCGACAATCACATAAAATACTCCAGCC
AATATAAGCTAGAGCATACTATCCGTTCGAGCAGGCAACCGGCGAAAGAAGTTAACTGGA
ACAGAAGGAAGCCGATGTCGGAACCTCGGATGCCTCATCGTGAAGAATCCAGCCAATGCT
ACAACAATCTGAATCGGTGTTATGAAGAAAACTATGGCCGCAATAAAGCACAATATAACA
AATATCGCGGTAGCGCGTGGATCTCTCCAGCTTAAAAGTGCTTGAAAACGTTCTCCTTGT
GTTGCAAGATCTCCGATCACGGTCTGAATCCTCCCGGCCACGCTTCTAAGCCGGTCATAC
CTTAAACGCACCATGTCAGGGTTTCTAGTGGTTGGAAACGTATCAAACTCTTCATCAAGC
TCGTCTGGATGGACCGCCTCGGCTTGAGAGATTTTTGTGTTCATGTGTGGTGGATAACGT
GGTCTAAACCGGTAGTTCCAGAGCCCGATCAAAAACATGTAGAGAAACATTGTTGGTAAG
ATCAGTTCTGGGAGACAAACAAGCATCAAAAACAGAACATGAACAAGAACGGTCGTGATC
GGGTTTCGCCACGAGCAAATGTCCGAGAACCATTTTCCAACCGCGATAACACCGGAGAAA
ACTGTCATCATCCGGAAGAAATTAGCTTTGCTCTTACGCATACTCCACAAATGCGAATCC
GTCAATCTCTAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTG
GGATTGTGCGTCATCCCTTACGTCAGT
   
Sequence Name 59-K118683-0022-147-G08C-3144
GenBank Accession LN510112 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K118683-0022-147-G08C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTAGAGCTTGTACGGATTC
GCATTTGTGGAGTATGCGTAAGAGCAAAGCTAATTTCTTCCGGATGATGACAGTTTTCTC
CGGTGTTATCGCGGTTGGAAAATGGTTCTCGGACATTTGCTCGTGGCGAAACCCGATCAC
GACCGTTCTTGTTCATGTTCTGTTTTTGATGCTTGTTTGTCTCCCAGAACTGATCTTACC
AACAATGTTTCTCTACATGTTTTTGATCGGGCTCTGGAACTACCGGTTTAGACCACGTTA
TCCACCACACATGAACACAAAAATCTCTCAAGCCGAGGCGGTCCATCCAGACGAGCTTGA
TGAAGAGTTTGATACGTTTCCAACCACTAGAAACCCTGACATGGTGCGTTTAAGGTATGA
CCGGCTTAGAAGCGTGGCCGGGAGGATTCAGACCGTGATCGGAGATCTTGCAACACAAGG
AGAACGTTTTCAAGCACTTTTAAGCTGGAGAGATCCACGCGCTACCGCGATATTTGTTAT
ATTGTGCTTTATTGCGGCCATAGTTTTCTTCATAACACCGATTCAGATTGTTGTAGCATT
GGCTGGATTCTTCACGATGAGGCATCCGAGGTTCCGACATCGGCTTCCTTCTGTTCCAGT
TAACTTCTTTCGCCGGTTGCCTGCTCGAACGGATAGTATGCTCTAGCTTATATTGGCTGG
AGTATTTTATGTGATTGTCGGATATAGACTTATGTTACTATTTGTAATCTGGATTTGTTT
TATCTACAATTTTTTTTTCATTTGAAAACGTTATAATATTTTGGATGGTATTCATTAGTG
TTTCCTTTTCAAAAATACCTCTNAGAAAATCAGAGAAAGGATCA

Last Updated on 06.12.2018 16:11