Sequences from confirmation amplicon


Confirmation sequences for line 148E03 and hit At1g08390 are shown.
Line ID 148E03
Locus At1g08390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2642938
Primers used for PCR 03do - 8474, show primer details
   
Sequence Name 25-K109936-0022-148-E03C-03do
GenBank Accession LN497488 [GenBank]
Sequencing primer gene specific primer, 03do: 5'-GATTGAGAGGAGCTCTAAGTCGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K109936-0022-148-E03C-03do
GTTTGGATTTCTACTTGTCTCCTCAACTTGTGGAACAATTTTGTAGGTCTTACTAGTAAT
GAATGCCTTTTTGTAAAATTGTATCAGTATTTGAGTCCATGATCAAGAAGATGAGAAGTG
CAGCAGAAATTGGATAAATTGAAAACTAGCAATGGTGTGTTAAAAATGATACAACCTTGC
TTTTGATTCCGTAGAAGTACATACCCATGACTCTAAAGATCATCTTCAATAAACAAAAAA
AGGATTAATCTGGTCTGGTGAAACTAAAAGAAGAATAGATAAGTTAAGTGAAGTGAAGAA
TCAAGAGAACTCTTGGATCAAGGGCTCATAGAAGAGTCTGTATGCGTCCATGACTTCTAA
ATACCACATGGCTTCACGATCTGGGCTCACCGACAGGTCAACCATCTTATGAACCTGGTT
TCAGTTTAAAACAACAATGACAAGTTTCAGTAATCAAAGCATTGCAAATATATTCAATTG
TAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 09-K109935-0022-148-E03C-8409
GenBank Accession LN490226 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K109935-0022-148-E03C-8409
ATTTACAATTGAATATATTTGCAATGCTTTGATTACTGAAACTTGTCATTGTTGTTTTAA
ACTGAAACCAGGTTCATAAGATGGTTGACCTGTCGGTGAGCCCAGATCGTGAAGCCATGT
GGTATTTAGAAGTCATGGACGCATACAGACTCTTCTATGAGCCCTTGATCCAAGAGTTCT
CTTGATTCTTCACTTCACTTAACTTATCTATTCTTCTTTTAGTTTCACCAGACCAGATTA
ATCCTTTTTTTGTTTATTGAAGATGATCTTTAGAGTCATGGGTATGTACTTCTACGGAAT
CAAAAGCAAGGTTGTATCATTTTTAACACACCATTGCTAGTTTTCAATTTATCCAATTTC
TGCTGCACTTCTCATCTTCTTGATCATGGACTCAAATACTGATACAATTTTACAAAAAGG
CATTCATTACTAGTAAGACCTACAAAATTGTTCCACAAGTTGAGGAGACAAGTTAGAAAT
ACCAAACAACTTTAGACAAGTATTTCCACTTCGACTTAGAGCTCCTCTCAAT

Last Updated on 06.12.2018 16:11