Sequences from confirmation amplicon


Confirmation sequences for line 148E10 and hit At1g52825 are shown.
Line ID 148E10
Locus At1g52825 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19670713
Primers used for PCR 44oh - 3144, show primer details
   
Sequence Name 90-K114716-0022-148-E10C-44oh
GenBank Accession LN515058 [GenBank]
Sequencing primer gene specific primer, 44oh: 5'-TACCTTTGGTTTATTTTTGGTTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K114716-0022-148-E10C-44oh
CAATTGTATTTTACTTTTTAAAACATTAGCGTTTGTCGTCGATTTGATCCACTTCATTGT
CACAGTTCATCAATTTCAATCCAAAAAACCCCAAATTAATCAAGACCCCTAAAAGCTTGC
TCATCGTGTGTTCTATGTGAATCACATCAAGATATTGGAGTCTCATACAAATGTCATCAG
TAGGAACATCAAAGGGGATTCTAGAAATCGCCAAATTCGGTGTATACGTCGCAGTACCAA
TCGTCCTAATCTATACATTCGCCAACAACAGCACCAACATCAAGAAATTCATGGGCAATC
ATTCATACGTTGTTTATCCTAAAGAAGCTCCTCGTCCTCCTTCACCTGAAGAAAAAGAAA
TGAGTAATTACCAATATGGAGCCCCCATTTGAAGCCGACTGCACTATAGCAGCGGAGGGG
TTGGATCGACCTCGAGGGGGGGCCCGGTACCGTGAACGTCGGCTCGATTGTACCTGCGTT
CAAATACTTTGCGATCGTGTTGCGCGCCTGCCCGGTGCGTCGGCTGATCTCACGGATCGA
CTGCTTCTCTCGCAACGCCTTCCGACGGATGATGTTTAAAAGTCCCATGTGGATCACTCC
GTTGCCCCGTCGCTCACCGTGTTGGGGGGAAGGTGCACATGGCTCAGTTCTCAATGGAAA
TTATCTGCCTAACCGGCTCAGTTCTGCGTAGAAACCAACATGCAAGCTCCACCGGGTGCA
AAGCGGCAGCGGCTCAAACACTGATNGTTTAAACCGAAGGCGGGAAACGACAATCTGATC
CCCGGGTACCCTGTCCTCTCCAATGAAATGAACTTCCTTATATNGAGGAAGGGTCTTGCG
AAGGATAGTGGGATTGTGCGTCATCCC
   
Sequence Name 42-K114715-0022-148-E10C-3144
GenBank Accession LN504561 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K114715-0022-148-E10C-3144
TTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGAGCCGCTGCCGCT
TTGCACCCGGTGGAGCTTGCATGTTGGTTTCTACGCAGAACTGAGCCGGTTAGGCAGATA
ATTTCCATTGAGAACTGAGCCATGTGCACCTTCCCCCCAACACGGTGAGCGACGGGGCAA
CGGAGTGATCCACATGGGACTTTTAAACATCATCCGTCGGATGGCGTTGCGAGAGAAGCA
GTCGATCCGTGAGATCAGCCGACGCACCGGGCAGGCGCGCAACACGATCGCAAAGTATTT
GAACGCAGGTACAATCGAGCCGACGTTCACGGTACCGGGCCCCCCCTCGAGGTCGATCCA
ACCCCTCCGCTGCTATAGTGCAGTCGGCTTCAAATGGGGGCTCCATATTGGTAATTACTC
ATTTCTTTTTCTTCAGGTGAAGGAGGACGAGGAGCTTCTTTAGGATAAACAACGTATGAA
TGATTGCCCATGAATTTCTTGATGTTGGTGCTGTTGTTGGCGAATGTATAGATTAGGACG
ATTGGTACTGCGACGTATACACCGAATTTGGCGATTTCTAGAATCCCCTTTGATGTTCCT
ACTGATGACATTTGTATGAGACTCCAATATCTTGATGTGATTCACATAGAACACACGATG
AGCAAGCTTTTAGGGGTCTTGATTAATTTGGGGTTTTTTGGATTGAAATTGATGAACTGT
GACAATGAAGTGGATCANATCGACGACNAACGCTNATGTTTATAANAAGTAAAATACAAT
TGTTGAATAATTAAAAAAAAATCGAGAAAGAAAACTAAACCGAACCA

Last Updated on 06.12.2018 16:11