Sequences from confirmation amplicon


Confirmation sequences for line 148E10 and hit At4g20200 are shown.
Line ID 148E10
Locus At4g20200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10910391
Primers used for PCR BK90 - 8474, show primer details
   
Sequence Name 11-K105704-0022-148-E10C-BK90
GenBank Accession LN491112 [GenBank]
Sequencing primer gene specific primer, BK90: 5'-TCGTTTGAAGGCCACTAATACTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K105704-0022-148-E10C-BK90
GCAGTTCAGAAATTGCCAACTTCTGAATGGACTCATTATGGCCACTCGATTTCCATCGAT
GTCTCGGTGAGTCCATATATATAATACATATGAAAAGTTAGTGTATATTGATGTAGAGAA
GGTTAATACATGTGTATGTACATAATTTACCTTTTAGGAAATGGATGCCCTTAGAAAAGA
GATCGATGCACTAAAGCCGATACTAAAGAGCACACTCATGTCCTTCAAAGGCATTGACTC
GACAAAGAAGAGAATACTTATGATATATTTGTTGGTGAGCCTTGGCCTTGCATATCACTT
CGAGGAAGAGATCTATGAAACTCTAAAAATGAGTTTTGAAAACATAGATNAGATGATGGA
CGGTGAAGATGATCTGTACACAGTTTCCATCATCTTTTGGGTTTTCAGAAGACACGGTTA
CCACATATCTNACGGTAAAGGAGTCTCTCTACCTAAGCTTTTGTTCATCATTGACGTTGG
AACAAGTGTTTTTTTTTTAATCTCTAATTGTTATGTATAAATGACTTTTATTTATATTTT
GGTCAATAGGTGTATTCCAAAGATTCAAAGGTAGCATTGGAAACTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAAATTC
   
Sequence Name 27-K105705-0022-148-E10C-8409
GenBank Accession LN498386 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K105705-0022-148-E10C-8409
CTTTGATCTTTGGATACACCTATTGACCAAATATAAATAAAAGTCATTTATACATAACAA
TAAGAGATTAAAAAAAAAACACTTGTTCCAACGTCAATGATGAACAAAAGCTTAGGTAGA
GAGACTCCTTTACCGTAAGATATGTGGTAACCGTGTCTTCTGAAAACCCAAAAGATGATG
GAAACTGTGTACAGATCATCTTCACCGTCCATCATCTTATCTATGTTTTCAAAACTCATT
TTTAGAGTTTCATAGATCTCTTCCTCGAAGTGATATGCAAGGCCAAGGCTCACCAACAAA
TATATCAAAAGTATTCTCTTCTTTGTCGAGTCAATGCCTTTGAAGGACATGAGTGTGCTC
TTTAGTATCGGCTTTAGTGCATCGATCTCTTTTCTAAGGGCATCCATTTCCTAAAAGGTA
AATTATGTACATACACATGTATTAACCTTCTCTACATCAATATACACTAACTTTTCATAT
GTATTATATATATGGACTCACCGAGACATCGATGGAAATCGAGTGGCCATAATGAGTCCA
TTCAAAAGTTGGCAATTTCTTGAACTTGCGAATACTTTCTTGATCATCAAATGTCAAAGT
ATTAG
Primers used for PCR 43xd - 3144, show primer details
   
Sequence Name 34-K119006-0022-148-E10C-43xd
GenBank Accession LN501612 [GenBank]
Sequencing primer gene specific primer, 43xd: 5'-TAGTATACCTTTCCAGGCTGTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K119006-0022-148-E10C-43xd
TGTCATATAGAAGTGCTAAACCGGNTGAAGTACTGAATCATCATAATCCTTTCACGTGAG
AGTTGTGGCGAGAAGAACACTGCTTGCACAAGGAAATGATGTTCGACTATTCTGTCTCTG
AAGTAAGGTGGCAAATTAGTTGCAAAATCTAGCTCCTTGTACCATCTTGCATCCAAATAT
ATAGATATGTAGTCAGAATTAATCCTTTAAATGATTGTAATTAAGTTATAAGCTATATAA
ATTGTGTATAAATACTTTGTAAGGGTTTTGAGTTCTTGAAGGTATTGTAGTTGTCCTAAC
TTGAAGCTGAGCTTGGCAAACTTGAGTAGCATCTCGTCATGCTCTATTTCTTGTTCATAG
AACGGGATGAATTCCACTGGGACTAACATCTCCATGTTCCAATGCTGAGATAGACCAAGA
GCATTTCGTATACGCACTGAGAGATGAGGCGGGCATGTACCACTTGCAGCTAGTGACTCC
AAGTGGCTCGATGTGAAGCTCAACGCTTCCTCCAGTATAAAGTCTTTCGTTGTCCCCAAA
TTCGCAGCTTCATACAAGCTCAACATACCCTTGGCATCTCGAGTAAGAGATTCTTTGAAG
TTTCCATTGATCAGCAATGAAATCTACATATTACAACAGGTGCTGACGCGGGCATAGCCC
AGCAGGCCAGCGGCGGCGCTCTTGTTCATGGCGTAATGTCTCCGGTTCTAGTCGCAAGTA
TTCTACTTTATGCGACTAANACACGCGACAAGAAAACGCCAGGAAAAGGGCAGGGCGGCA
GCCTGTCGCGTAACTTAGGACTTGTGCGACATGTCGTTTTCAGAAGACGGCTGCACTGA
   
Sequence Name 04-K119005-0022-148-E10C-35St
GenBank Accession LN488130 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K119005-0022-148-E10C-35St
CTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATT
GTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGCAGCCGTCTTCTGAAAACGACATGTCG
CACAAGTCCTAAGTTACGCGACAGGCTGCCGCCCTGCCCTTTTCCTGGCGTTTTCTTGTC
GCGTGTTTTAGTCGCATAAAGTAGAATACTTGCGACTAGAACCGGAGACATTACGCCATG
AACAAGAGCGCCGCCGCTGGCCTGCTGGGCTATGCCCGCGTCAGCACCTGTTGTAATATG
TAGATTTCATTGCTGATCAATGGAAACTTCAAAGAATCTCTTACTCGAGATGCCAAGGGT
ATGTTGAGCTTGTATGAAGCTGCGAATTTGGGGACAACGAAAGACTTTATACTGGAGGAA
GCGTTGAGCTTCACATCGAGCCACTTGGAGTCACTAGCTGCAAGTGGTACATGCCCGCCT
CATCTCTCAGTGCGTATACGAAATGCTCTTGGTCTATCTCAGCATTGGAACATGGAGATG
TTAGTCCCAGTGGAATTCATCCCGTTCTATGAACAAGAAATAGAGCATGACGAGATGCTA
CTCAAGTTTGCCAAGCTCAGCTTCAAGTTAGGACAACTACAATACCTTCAAGAACTCAAA
ACCCTTACAAAGTATTTATACACAATTTATATAGCTTATAACTTAATTACAATCATTTAA
AGGATTAATTCTGACTACATATCTATATATTTGGATGCAAGATGGTACAAGGAGCTAGAT
TTTGCAACTAATTTGCCACCTTACTTCAGAGACAGAATAGTCGAACATCAATTTCTTGTG
CAAGCAGTGTTCTTTCTCGCACAACTC

Last Updated on 06.12.2018 16:11