Sequences from confirmation amplicon


Confirmation sequences for line 149D06 and hit At1g19330 are shown.
Line ID 149D06
Locus At1g19330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6682384
Primers used for PCR 04um - 8474, show primer details
   
Sequence Name 40-K117387-0022-149-D06C-04um
GenBank Accession LN503850 [GenBank]
Sequencing primer gene specific primer, 04um: 5'-TGTTACTTCCATAGGAATTCTGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K117387-0022-149-D06C-04um
GTAAACTAATCGTGATACTGAAGAAATTCGTAAATTATATGGATTATTCTCTAAAGTTTA
AAGCTAGAATATGGTGAAGTTACTCCTTTAGGTCCACATACACAGAGACACTATCAAAAA
GATCTTGCAGAGCAGTTTATAATATAAACTAGCCAACCAAAGCATAAAGATTTTATAAAG
AATAGTAATCGACAGAAGTGACACTGCTTGGGGACGAAAATGACTCAACTAATTAAGAAA
GAACAAAAAAGAAGAAAAACCTCTAAAACAAATGATTAACATTAATCAAAAGACTAACAA
ATTTCAAAAAGCCAGCTTGGATCCTTAAGGTGGAGAACAAAAATGTCAAGAAATATGATG
GAGAATTAGATAACAAGTGCATATTTGTTATGATGTTTTATTGACCAGGCGCCAAAATAA
AGTATGAGTATGATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCG
   
Sequence Name 13-K117386-0022-149-D06C-8409
GenBank Accession LN492228 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K117386-0022-149-D06C-8409
TTGAATCATACTCATACTTTATTTTGGCGCCTGGTCAATAAAACATCATAACAAATATGC
ACTTGTTATCTAATTCTCCATCATATTTCTTGACATTTTTGTTCTCCACCTTAAGGATCC
AAGCTGGCTTTTTGAAATTTGTTAGTCTTTTGATTAATGTTAATCATTTGTTTTAGAGGT
TTTTCTTCTTTTTTGTTCTTTCTTAATTAGTTGAGTCATTTTCGTCCCCAAGCAGTGTCA
CTTCTGTCGATTACTATTCTTTATAAAATCTTTATGCTTTGGTTGGCTAGTTTATATTAT
AAACTGCTCTGCAAGATCTTTTTGATAGTGTCTCTGTGTATGTGGACCTAAAGGAGTAAC
TTCACCATATTCTAGCTTTAAACTTTAGAGAATAATCCATATAATTTACGAATTTCTTCA
GTATCACGATTATGTTTTACTATTAGTGATATCACATTCCACCAGTTCTTGTCTCCCAGA
ATTCCTATGAAAGTAAC

Last Updated on 06.12.2018 16:11