Sequences from confirmation amplicon


Confirmation sequences for line 150A08 and hit At1g58080 are shown.
Line ID 150A08
Locus At1g58080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21506981
Primers used for PCR 23de - 8474, show primer details
   
Sequence Name 29-K109778-0022-150-A08C-23de
GenBank Accession LN499331 [GenBank]
Sequencing primer gene specific primer, 23de: 5'-GTTGCATAAATCCTACACCCACTT-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K109778-0022-150-A08C-23de
TATTCTTTCTTTACGTTTTTTCTATAAAGATTCCACTAAAATAAGAAAAGCCACCACAAA
ACATATAAAGGAAAAGTGGGTTTGGGGTTTTTTTTTTAAATGGGCCTTAAAAATTTTATT
GTTGAATTTGGCCCTTCCAAAAAGAAAGGGGTTTTGGGTTTTTTTGTTAAATATTTTTCT
TTGGGGGGATAAAAAAAATCTTTTTCTTTTTGAAAATCCCAAAAAAAGGGTTTTTTTTTT
TGGCTAAGATTTTTAAAAAAACCCTTTTTTTTTTTTTTTTTCCTTTTTAAAGATTGTTTT
TTTTGCGCCTTTTTTCGTGGGTTTTTTCTTTTTTTGAACCTGTGGGGGATTTTTCGAAGT
GGGTTTTTTTTGGGGTCCCCCGGTTTAAAAGTTGCTTTGAAAAAAGTGGTTTTTTTTAAT
TTTTTTGTTGGGGTTTTTTTTGCTTTGGGGGGAAAAAATTTTTCTTTGGTTTTTCTTAAA
AAGGGATTTGGGGGGGTGTAAATATTTATTTTTTCGGGGGTGGAATTTTT
   
Sequence Name 13-K109777-0022-150-A08C-8409
GenBank Accession LN492083 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K109777-0022-150-A08C-8409
GCCATTTACATTGAACCAGAAACAGAGAGAGCTAATATAAAAATCATAAATCAGTCTCTA
TCAAAATCTCAATGACGAAACATTTCTCAGTCTCTAAGAACTAAATACAAGCTGAGTGAA
GCTAAACAATTAAATCTGAGATTAACAATCGCAATGTTAAATCTCAGACCTTGAGAAGAT
CGATTGCATCAGCAGCCATACGTCCTTTACTAGGAAGACCAAGACGAATCTGGTCACGCT
CAACGACAACAGCGGCCGGAGCAGGAGCTGGAGAGGAACCGTTTGTTGCGACGGAGCTTT
GAACGGTGGAGACACAAGAAGTAACCAATCTGAGACATCTCCGACGAGGTACGATGACGG
AGAAAGCGGTGGAAGGAGGCGGAGATAGGATAGGTGTTGAAGATGGGAAGGAGGAAGAAG
AAGG

Last Updated on 06.12.2018 16:11