Sequences from confirmation amplicon


Confirmation sequences for line 151G05 and hit At1g03290 are shown.
Line ID 151G05
Locus At1g03290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:810678
Primers used for PCR 15bk - 8474, show primer details
   
Sequence Name 43-K107839-0022-151-G05C-15bk
GenBank Accession LN504858 [GenBank]
Sequencing primer gene specific primer, 15bk: 5'-AGAGGTTTGAAGAAGAGTTTGCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K107839-0022-151-G05C-15bk
TGAGAGAGAGACAGCATTGGTTGCCAAGGAGAAGAAAGAAGCGGCTGACAAAAGGATTGG
TGAAATGAAGACATGTGCAGAAACCATTGATCAAGAGATCAAAGATGAGGAGCTTGAGTT
TCAGACAACTGTCTCGACTCCATGGTAATAATAAGATTTAGAGTAGCTCCATGGTAATAA
GATTTAGAGTAGATGTATGTTAAAACAATTATCTACGATCTTTATTTCCGTTTGTTTATT
GTTGATTATGCATTGAACCATCTTTTGAAGAGATTAGAATAATCTAACAGGTTTATGGAT
CATTTAACGTCCCTTTTACTAAAAAATTTCTTAAAACATTGGCTTTGTCAAACAACCACA
AAAAGACTGAGAAAATCCATAAGTTAAATAAAGCTAGAACCACATATAAATCCCAATCCA
AGTTTTGGTCTAAAAGAGTAGGAAACCAATCCATTTATTTTTAGCATTTAAGTACCCTAT
ATGTACAATAACTTCATTCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 75-K107840-0022-151-G05C-8409
GenBank Accession LN513252 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K107840-0022-151-G05C-8409
ATTTACATTGAAGAATGAAGTTATTGTACATATAGGGTACTTAAATGCTAAAAATAAATG
GATTGGTTTCCTACTCTTTTAGACCAAAACTTGGATTGGGATTTATATGTGGTTCTAGCT
TTATTTAACTTATGGATTTTCTCAGTCTTTTTGTGGTTGTTTGACAAAGCCAATGTTTTA
AGAAATTTTTTAGTAAAAGGGACGTTAAATGATCCATAAACCTGTTAGATTATTCTAATC
TCTTCAAAAGATGGTTCAATGCATAATCAACAATAAACAAACGGAAATAAAGATCGTAGA
TAATTGTTTTAACATACATCTACTCTAAATCTTATTACCATGGAGCTACTCTAAATCTTA
TTATTACCATGGAGTCGAGACAGTTGTCTGAAACTCAAGCTCCTCATCTTTGATCTCTTG
ATCAATGGTTTCTGCACATGTCTTCATTTCACCAATCCTTTTGTCAGCCGCTTCTTTCTT
CTCCTTGGCAACCAATGCTTGTCTCTTCTTCTCAATAATTATGCGTTCAAAGTCAGCAAA
CTCTTCTTCAAACCTC

Last Updated on 06.12.2018 16:11