Sequences from confirmation amplicon


Confirmation sequences for line 151G06 and hit At5g24150 are shown.
Line ID 151G06
Locus At5g24150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8174926
Primers used for PCR 58xc - 8474, show primer details
   
Sequence Name 41-K119110-0022-151-G06C-58xc
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 58xc: 5'-GACTTGGACAGTGTTCTACGTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K119110-0022-151-G06C-58xc
GAGAAACGGTGCTACTGACGTTATCATCGTTGGGGCTGGAGTAGGCGGCTCGGCTCTCGC
ATATGCTCTTGCTAAGGTATGTAAAGTGGCGTCACAGTTTCACAGATTTGCGTCTTAGTA
TTAAATTGATTCTTGTTGTTTTTGTATAATATTTGTGCATGAGTACTGATAAAGGTTTGA
ACTATATACACTATATATGATTGTTGTTGTTTTTTTGCATTATACACTTCGGCTGCAATA
TTTTTGGGTTGGAACGAATATATTCGTATGCTAATTTCCGATTATAAATAGGACGGGCGT
CGAGTCCATGTAATAGAGAGGGACCTGAGAGAACCAGAGAGAATCATGGAAAGTTAAAGC
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
AGCACGGTTATTA
   
Sequence Name 18-K119109-0022-151-G06C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K119109-0022-151-G06C-8409
ATTGGCTTTACTTTCATGATTCTCTCTGGTTCTCTCAGGTCCCTCTCTATTACATGGACT
CGACGCCCGTCCTATTTATAATCGGAAATTAGCATACGAATATATTCGTTCCAACCCAAA
AATATTGCAGCCGAAGTGTATAATGCAAAAAAACAACAACAATCATATATAGTGTATATA
GTTCAAACCTTTATCAGTACTCATGCACAAATATTATACAAAAACAACAAGAATCAATTT
AATACTAAGACGCAAATCTGTGAAACTGTGACGCCACTTTACATACCTTAGCAAGAGCAT
ATGCGAGAGCCGAGCCGCCTACTCCAGCCCCAACGATGATAACGTCAGTAGCACCGTCTT
CTCGCTCTTCAACCACCGCATCCGCCAACTGCGTCGCCTTCTTCCCCCTGTTTGTGACGT
AGAACATGCTCCAAGT

Last Updated on 06.12.2018 16:11