Sequences from confirmation amplicon


Confirmation sequences for line 151G12 and hit At4g11080 are shown.
Line ID 151G12
Locus At4g11080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6761693
Primers used for PCR KM71 - 8474, show primer details
   
Sequence Name 08-K101221w-0022-151-G12C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K101221w-0022-151-G12C-8409
ATTTACATTGAATATATCCTGACAAGTTTGTTAAGTTCATCCATACTCATCATAGACATT
GATGTGTCAAGAAGTGAAGTTTAATAAAGGGATGTTCTTAACGTATGTGTAACAACAGAT
GGCGAAAAAGAACAAGGAGATATATCTGCAAGAAATGGAAGGATATAAAAGAACAAAGGA
AGAAGAAGCAATGAGCCAGAAGAAAGAAGAAGAAGAATTCATGAAACTCCATAAACAAGA
AGCTCTCCAACTTCTCAAGAAGAAGGAGAAAACCGACAATATCATAAAGGTTAATATACT
AAACTCACAGTGCATTGAGCTATTTTGGGATATAAGTCAGTAACTAACACAACACATGTT
TTCTTTCTTGCAGAAGACGAAAGAGACGGCGAAGAACAAGAAAAAGAACGAGAATGTTGA
TCCTAACAAACCCAAAAAGCCTACTTCTTCATACTTTCTCTTCTGGTATATACTTTAAAA
CCCTGAATCCACAAAACAATCAGATGCTAAACTGGTTTTATAATCTCAAATCTTTTTACA
GCAAAGACGCAAGAAAGAGTGTGTTAGAAGAACATCCTGGGATCAACAACCAAACTGTT
   
Sequence Name 72-K101228-0022-151-G12C-KM71
GenBank Accession LN512688 [GenBank]
Sequencing primer gene specific primer, KM71: 5'-TAACAGTTGAGTTGTTGATCCCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K101228-0022-151-G12C-KM71
CTGTAAAGATTTGAGATTTAAAACCAGTTTAGCATCTGATTGTTTTGTGGATTCAGGGTT
TTAAAGTATATACCAGAAGAGAAAGTATGAAGAAGTAGGCTTTTTGGGTTTGTTAGGATC
AACATTCTCGTTCTTTTTCTTGTTCTTCGCCGTCTCTTTCGTCTTCTGCAAGAAAGAAAA
CATGTGTTGTGTTAGTTACTGACTTATATCCCAAAATAGCTCAATGCACTGTGAGTTTAG
TATATTAACCTTTATGATATTGTCGGTTTTCTCCTTCTTCTTGAGAAGTTGGAGAGCTTC
TTGTTTATGGAGTTTCATGAATTCTTCTTCTTCTTTCTTCTGGCTCATTGCTTCTTCTTC
CTTTGTTCTTTTATATCCTTCCATTTCTTGCAGATATATCTCCTTGTTCTTTTTCGCCAT
CTGTTGTTACACATACGTTAAGAACATCCCTTTATTAAACTTCACTTCTTGACACATCAA
TGTCTATGATGAGTATGGATGAACTTAACAAACTTGTCAGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAN
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATNTCCGC

Last Updated on 06.12.2018 16:11