Sequences from confirmation amplicon


Confirmation sequences for line 152F03 and hit At3g55290 are shown.
Line ID 152F03
Locus At3g55290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20503043
Primers used for PCR 54wj - 8474, show primer details
   
Sequence Name 92-K118629-0022-152-F03C-54wj
GenBank Accession LN515214 [GenBank]
Sequencing primer gene specific primer, 54wj: 5'-TCGATCTGGTAATGAAGTAGGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K118629-0022-152-F03C-54wj
ACCCTACGATTTGGGCATAACCGTCCTTTGTTATGGGACGTGTTCTACTTGGTATCTCTA
GAACCGCGNGTCTTTGGCAGATGGATCAGATTATATCTTTTTGACACTTGTATTTATATA
TGTTATATGTTATTGGCTTCTCCTTCCCCATTGGCCATCGAACACACCACACAGAGAAAA
CAATATGAGCAATCATCAAACTGTGAGATAACATTTTCTCTCATTTCCCATCTTTCTTTT
TATCTTCAAAACTATTCATATAACCATTTATATAAGACCACTCTCCAAATTACCATGCTT
TTAACCTTTAAATATGATTTCTGTTATCTTCTTTACCATATGATTCCATATGGAAAAATT
GTCAATCATGTGTTTTTGTCTTTGTAATCTGAAATTACTTGATTTTTGTATTTTGTTTTT
TTTTTGGGGGAGAAAAGCAGGTGTTGAAGCTATTGGAGCCATGGTGTGAACTTAAAGACA
AAGTGGTTCTTGTGACAGGAGCTTCCTCTGGTATTGGAAGAGAGATCTGTCTTGATCTTG
CCAAAGCTGGTTGCCAGGTTATTGCAGCAGCTCGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGC
   
Sequence Name 73-K118628-0022-152-F03C-8409
GenBank Accession LN512977 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K118628-0022-152-F03C-8409
CCATTACGAGCTGCTGCAATAACCTGGCAACCAGCTTTGGCAAGATCAAGACAGATCTCT
CTTCCAATACCAGAGGAAGCTCCTGTCACAAGAACCACTTTGTCTTTAAGTTCACACCAT
GGCTCCAATAGCTTCAACACNCTGCTTTTCTCCCCCAAAAAAAAAACAAAATACAAAAAT
CAAGTAATTTCAAATTACAAAGACAAAAACACATGATTGACAATTTTTCCATATGGAATC
ATATGGTAAAAAAAATAACAAAAATCATATTTAAAGGTTAAAAGCATGGTAATTTGGAAA
GTGGTCTTATATAAATGGTTATATGAATAGTTTTGAAAATAAAAAGAAAGATGGGAAATG
AAAAAAAATGTTATCTCACAGTTTGATGATTGCTCATATTGTTTTCTCTGTGTGGTGTGT
TCAATGGCCAATGGGGAAGGAGAAGCCAATAACATATAACATATATAAATACAAGTGTCA
AAAAGATATAATCTGATCCATCTGCCAAAAACCGCGGTTCTAGAGATACCAAGTAAAACA
CGTCCCATAACAAAGGACGGTTTATGCCCAAATCTGTAGGGTCTAGGTCCTAATTAACAC
TTTAGTCTACTACATGTAAAGCCTACTTCATTACCAAATCAAA

Last Updated on 06.12.2018 16:11