Sequences from confirmation amplicon


Confirmation sequences for line 152H03 and hit At5g38570 are shown.
Line ID 152H03
Locus At5g38570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15449375
Primers used for PCR 13rn - 8474, show primer details
   
Sequence Name 26-K116083-0022-152-H03C-13rn
GenBank Accession LN498074 [GenBank]
Sequencing primer gene specific primer, 13rn: 5'-CAATTGCTCAAAGGTTCTCCTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K116083-0022-152-H03C-13rn
GTAGTTTTGATAATAAAAAAAAAAAATGTTTTGTTTTTCTGGTACCTAAAAGGGTTTTTA
ATCAAATTCTTCGGCTTGGTCTCAAAAATAATGAGCGTAATGATTTTGTTTGCTGGAATC
AACCGAGTTCTGTTCTTGAGTGCTTGTTGTCAAGTCTAAAAATATTGAACTGGTCACCAT
ATTTTGGGAAACCCCAAAATAGAGATTTTGCATTTTACATCTTGAAAAACCCTTGTCACT
TAAAAACTGCAACATTCTTGACTGATAAAAAAATAATTGAAAAAAAAAAAGCTACTTTTA
ATATGGATCACCAATGAGTATGATGGTCAATATGGAAAAAAAAAAAGAGTATTTCCCATT
TTTTTTTCATTTCAAAAATGTAAATTTCCC
   
Sequence Name 10-K116082-0022-152-H03C-8409
GenBank Accession LN490808 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K116082-0022-152-H03C-8409
TCTTTTATCAGTCAAGAATGTTGCAGTCTTTAAGTGACAAGCGTTTTTCAAGATGTAAAC
TGCAATATCTCTATCTTGTGGTCTCCCAAAATATGCTGACCAGTTCAATATCTTTAGACT
CGACAACAAGCACTCAAGAACAGAACTCGGTTGATTCCAGCAAACAATATCATTACGCTC
ATCATCTATGAGACCAAGCAGAAGAATTAGATTAAAAACCATATTAGGTACCAGAAAAAC
AAAACATTTTTTTTTTTTATTATCAAAACTTACTTTCATTTCAAAGATGTCTAAAACTTG
TAGGTTAGGAAAACCTTT

Last Updated on 06.12.2018 16:11