Sequences from confirmation amplicon


Confirmation sequences for line 152H10 and hit At4g18700 are shown.
Line ID 152H10
Locus At4g18700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10289387
Primers used for PCR JK05 - 8474, show primer details
   
Sequence Name 03-K024285-022-152-H10C-JK05
GenBank Accession LN487316 [GenBank]
Sequencing primer gene specific primer, JK05: 5'-CCTGAGGTTTTAGCTAGGAAAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K024285-022-152-H10C-JK05
TATGATGCTGCTAAAGTTGACATTTGGTCTTGTGGTGTTATCTTGTTTGTGTTGATGGCT
GGTTATTTGCCGTTTCATGATCGGAATGTTATGGCTATGTATAAGAAGATTTACAGAGGG
GAGTTTAGGTGTCCTAGGTGGTTTTCTACTGAGCTTACCAGGTTGTTGTCGAAGCTTTTG
GAGACGAATCCGGAGAAACGGTTCACTTTCCCTGAGATTATGGAGAATTCTTGGTTTAAG
AAAGGGTTTAAGCATATTAAGTTTTATGTGGAGGATGATAAGTTGTGTAATGTTGTTGAT
GATGATGAACTGGAGTCTGACTCGGTGGAGTCGGATAGAGATTCCGCGGCTTCTGAGTCG
GAGATTGAGTATTTGGAGCCTAGGAGGAGAGTTGGAGGGTTGCCTAGACCTGCGAGTTTG
AATGCTTTCGATATTATATCGTTTTCGCAAGGTTTTGATTTATCGGGTTTGTTTGATGAC
GATGGGGAGGGTTCTAGGTTTGTTTCGGGAGCTCCGGTTTCGAAGATTATATCGAAGTTG
GAAGAGATTGCTAAAGTTGTGAGCTTTACTGTGAGGAAGAAGGATTGTAGAATGTAGATC
TACCATCATGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
   
Sequence Name 04-K024285-022-152-H10C-8409
GenBank Accession LN487755 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K024285-022-152-H10C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATTCATGATGGTAGATCTACAT
TCTACAATCCTTCTTCCTCACAGTAAAGCTCACAACTTTAGCAATCTCTTCCAACTTCGA
TATAATCTTCGAAACCGGAGCTCCCGAAACAAACCTAGAACCCTCCCCATCGTCATCAAA
CAAACCCGATAAATCAAAACCTTGCGAAAACGATATAATATCGAAAGCATTCAAACTCGC
AGGTCTAGGCAACCCTCCAACTCTCCTCCTAGGCTCCAAATACTCAATCTCCGACTCAGA
AGCCGCGGAATCTCTATCCGACTCCACCGAGTCAGACTCCAGTTCATCATCATCAACAAC
ATTACACAACTTATCATCCTCCACATAAAACTTAATATGCTTAAACCCTTTCTTAAACCA
AGAATTCTCCATAATCTCAGGGAAAGTGAACCGTTTCTCCGGATTCGTCTCCAAAAGCTT
CGACAACAACCTGGTAAGCTCAGTAGAAAACCACCTAGGACACCTAAACTCCCCTCTGTA
AATCTTCTTATACATAGCCATAACATTCCGATCATGAAACGGCAAATAACCAGCCATCAA
CACAAACAAGATAACACCACAAGACCAAATATCAACTTTAGCAGCATC

Last Updated on 06.12.2018 16:11