Sequences from confirmation amplicon


Confirmation sequences for line 153C02 and hit At4g00820 are shown.
Line ID 153C02
Locus At4g00820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:350696
Primers used for PCR 11uw - 3144, show primer details
   
Sequence Name 68-K117671-0022-153-C02C-11uw
GenBank Accession LN512086 [GenBank]
Sequencing primer gene specific primer, 11uw: 5'-TAAGTTTCAGAACACCATTGTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K117671-0022-153-C02C-11uw
CATCAGCTATAAAAGCTAACATTTTGAAATGCCTTTTTTGTTGTTGTGTAGTCAAGAGAA
GGAAGTAGCATTGCGGAAGATTGGGATGATCGACCACACACGATTGAGGAAGTGAAAGCA
ATGTTGCAACAAAGACGAGACAATGCGTTGAGACGTGAGAGTAACAATAGTATATCACAA
GCTTTCTCTCACCAGGTAATAGATATGAATTCATATTAATCGTTTGTTTGTTTTCGGTAA
TTTGATTCGTTTCGGTGTTTGATTTCGGTGAAAGGTTCGGAGAACAAGAGGTAGTTATTC
TACAGGAGACGAGTATGAAGAAGAGAGACCGAAATGGTTAGACAGATGGATGGCTTCTAA
ACCGTGGGATAAACGAGCTTCAACGGATCAAAGAGTACCACCGGTTTACAAAACCGTGGA
GATCGATACTTCTCAACCGTGAGCCACCGGTTGTAAATGGCTTCATGTAAAGCGTTGTTT
TTACAGTCCACCGGTTGTAAGTTATGGACTCGCTAGTCAAACACTGATAGTTTAAACCGA
AGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTC
CTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAG
TGGAGATATCA
   
Sequence Name 20-K117670-0022-153-C02C-3144
GenBank Accession LN495393 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K117670-0022-153-C02C-3144
TCCACTATCTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAG
GGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAACTATCAGTGTTTGACTAG
CGAGTCCATAACTTACAACCGGTGGACTGTAAAAACAACGCTTTACATGAAGCCATTTAC
AACCGGTGGCTCACGGTTGAGAAGTATCGATCTCCACGGTTTTGTAAACCGGTGGTACTC
TTTGATCCGTTGAAGCTCGTTTATCCCACGGTTTAGAAGCCATCCATCTGTCTAACCATT
TCGGTCTCTCTTCTTCATACTCGTCTCCTGTAGAATAACTACCTCTTGTTCTCCGAACCT
TTCACCGAAATCAAACACCGAAACGAATCAAATTACCGAAAACAAACAAACGATTAATAT
GAATTCATATCTATTACCTGGTGAGAGAAAGCTTGTGATATACTATTGTTACTCTCACGT
CTCAACGCATTGTCTCGTCTTTGTTGCAACATTGCTTTCACTTCCTCAATCGTGTGTGGT
CGATCATCCCAATCTTCCGCAATGCTACTTCCTTCTCTTGACTACACAACAACAAAAAAG
GCATTTCANAATGTTAGCTTTATATAGCTGATTGCTGAAACAGAGCANAAATGGTCGTTG
AAACAGAGCAACAATGGTG

Last Updated on 06.12.2018 16:11