Sequences from confirmation amplicon


Confirmation sequences for line 153E12 and hit At1g53500 are shown.
Line ID 153E12
Locus At1g53500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:19968933
Primers used for PCR MP01 - 8474, show primer details
   
Sequence Name 57-K015146-022-153-E12C-8409
GenBank Accession LN509257 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K015146-022-153-E12C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTGAAACTTGTTTTTGAG
TTTACCAAGAACAATATTTATGGTACTCATGTTCTTTTGGAAGCCTGTAAAGTTACAGGA
CAGATCAGGAGGTTTATCCATGTGAGTACCGATGAAGTCTATGGAGAAACCGATGAGGAT
GCTGCTGTAGGAAACCATGAAGCTTCTCAGCTGTTACCGACGAATCCTTACTCTGCAACT
AAGGCTGGTGCTGAGATGCTTGTGATGGCTTATGGTAGATCATATGGATTGCCTGTTATT
ACGACTCGCGGGAACAATGTTTATGGGCCTAACCAGTTTCCTGAAAAAATGATTCCTAAG
TTCATCTTGTTGGCTATGAGTGGGAAGCCGCTTCCCATCCATGGAGATGGATCTAATGTC
CGGAGTTACTTGTACTGCGAAGACGTTGCTGAGGCTTTTGAGGTTGTTCTTCACAAAGGA
GAA
   
Sequence Name 61-K015146-022-153-E12C-MP01
GenBank Accession LN510385 [GenBank]
Sequencing primer gene specific primer, MP01: 5'-ATTTCTCCTTTGTGAAGAACAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K015146-022-153-E12C-MP01
AAAACCTCAGCAACGTCTTCGCAGTACAAGTAACTCCGGACATTAGATCCATCTCCATGG
ATGGGAAGCGGCTTCCCACTCATAGCCAACAAGATGAACTTAGGAATCATTTTTTCAGGA
AACTGGTTAGGCCCATAAACATTGTTCCCGCGAGTCGTAATAACAGGCAATCCATATGAT
CTACCATAAGCCATCACAAGCATCTCAGCACCAGCCTTAGTTGCAGAGTAAGGATTCGTC
GGTAACAGCTGAGAAGCTTCATGGTTTCCTACAGCAGCATCCTCATCGGTTTCTCCATAG
ACTTCATCGGTACTCACATGGATAAACCTCCTGATCTGTCCTGTAACTTTACAGGCTTCC
AAAAGAACATGAGTACCATAAATATTGTTCTTGGTAAACTCAAAAACAAGTTTCAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCCAC
GTGTCTACAT

Last Updated on 06.12.2018 16:11