Sequences from confirmation amplicon


Confirmation sequences for line 154A02 and hit At2g35060 are shown.
Line ID 154A02
Locus At2g35060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14777764
Primers used for PCR BE05 - 8474, show primer details
   
Sequence Name 36-K028098-022-154-A02C-8409
GenBank Accession LN502091 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K028098-022-154-A02C-8409
ATTGAATATGTCCATGTAGATCCTCAAATACGTTTTCGTTGTATGTAAAGCAAATGATAA
TGGTCAAGGTAGTGTTTCCTCAATTCACAAGCTTTATTTCCTCTGTTTTTTATAATCGGT
GTTACTGACTTGATTGTCTTTGTAGGTGGGACGTTTGCTTTGTATTCATTACTCTGTAGA
CATGCCAAAGTCAAAACAATTCAGAACCAACACCGTACTGATGAAGAGCTTACTACTTAC
AGCCGCACTACGTTCCATGAGCATTCCTTTGCTGCTAAGACTAAAAGATGGTTAGAAAAG
CGGACATCTAGAAAAACGGCGCTTCTTATTTTAGTTCTTGTTGGTACGTGTATGGTCATC
GGCGATGGTATCCTTACTCCCGCTATTTCTGGTATGTAGTTCATTTTCCCTTTCAAGTAT
GTGATTTAGGCCTAAGAGTAATGAAAATGTTCACTTGTCAACATTTGCTTTTGCAGTTCT
CTCAGCTGCTGGTGGGCTAAGAGTAAACCTTCCTCACATAAGCAATGGTATTTAACATTT
TTACATGCGTTGAACTTAATCCTGAAACATCTCGTGGTGTCAATAATAATAGTCATAAGT
TTGGTGGTTACTATTACAGGTGTTGTTGTTTTTGTTGCTGTTGTGATATTAGTCAGCTTG
TTTAGCGT
   
Sequence Name 36-K028098-022-154-A02C-BE05
GenBank Accession LN502092 [GenBank]
Sequencing primer gene specific primer, BE05: 5'-CTGTTCCATAATGCTGTACGCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K028098-022-154-A02C-BE05
GCACAAAACACACACCTGTAATAGTAACCACCAAACTTATGACTATTATTATTGACACCA
CGAGATGTTTCAGGATTAAGTTCAACGCATGTAAAAATGTTAAATACCATTGCTTATGTG
AGGAAGGTTTACTCTTAGCCCACCAGCAGCTGAGAGAACTGCAAAAGCAAATGTTGACAA
GTGAACATTTTCATTACTCTTAGGCCTAAATCACATACTTGAAAGGGAAAATGAACTACA
TACCAGAAATAGCGGGAGTAAGGATACCATCGCCGATGACCATACACGTACCAACAAGAA
CTAAAATAAGAAGCGCCGTTTTTCTAGATGTCCGCTTTTCTAACCATCTTTTAGTCTTAG
CAGCAAAGGAATGCTCATGGAACGTAGTGCGGCTGTAAGTAGTAAGCTCTTCATCAGTAC
GGTGTTGGTTCTGAATTGTTTTGACTTTGGCATGTCTACAGAGTAATGAATACAAAGCAA
ACGTCCCACCTACAAAGACAATCAAGTCAGTAACACCGATTATAAAAAACAGAGGAAATA
AAGCTTGTGAATTGAGGAAACACTACCTTGACCATTATCATTTGCTTTACATACAACGAA
AACGTATTTGAGGATCTACATGGACATATTCAATTGTAAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTTATTTCGACGTG

Last Updated on 06.12.2018 16:11