Sequences from confirmation amplicon


Confirmation sequences for line 155A04 and hit At1g01900 are shown.
Line ID 155A04
Locus At1g01900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:310795
Primers used for PCR 14rn - 8474, show primer details
   
Sequence Name 61-K115963-0022-155-A04C-14rn
GenBank Accession LN510598 [GenBank]
Sequencing primer gene specific primer, 14rn: 5'-AACCATTAGTAAGGGCTCTCCATT-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K115963-0022-155-A04C-14rn
CAACAAATATTTAGTTAAAAGTCATCAAAAGCATTTTCATTTTGATCAATTATTTTTGAG
GAAGGTAATTTGATGTTCGGATCAACGTTATGGAGCTACCCAACGTTTTTAATTATATCT
TATGTGTTTATACGAGTTATATTTGCATAATATATATGGTAGAAGTTTTTTTTTGGTAAA
AGTTGGAAGTTATTCTTCACGTTATTCCCATTAACATTTGGAGACTAAACAATGTGAGAT
CAAAAGAAGTTAAAAAACAGAGGGCTCATTTATATTTCAACCGTCTAACCTGAAATCAGT
GGTTTCATTGATTTTTCCAACGATTGATTCGTAGCCTTTGTAAAATGCGCTAGCTCCAAT
GATCTTCTTGTTACACTCCGAAGATGAGAAGTTTGTGCCTTCATCGCACGAACCTCTCCA
TCTAGACGGTACAGGAGTCATGTGCGTGTCTCGGAAGCTCACGTGCTCCGGGGAGAATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCC
   
Sequence Name 61-K115962-0022-155-A04C-8409
GenBank Accession LN510597 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K115962-0022-155-A04C-8409
CATTTACATTGAATATATTCTCCCCGGAGCACGTGAGCTTCCGAGACACGCACATGACTC
CTGTACCGTCTAGATGGAGAGGTTCGTGCGATGAAGGCACAAACTTCTCATCTTCGGAGT
GTAACAAGAAGATCATTGGAGCTAGCGCATTTTACAAAGGCTACGAATCAATCGTTGGAA
AAATCAATGAAACCACTGATTTCAGGTTAGACGGTTGAAATATAAATGAGCCCTCTGTTT
TTTAACTTCTTTTGATCTCACATTGTTTAGTCTCCAAATGTTAATGGGAATAACGTGAAG
AATAACTTCCAACTTTTACCAAAAAAAAACTTCTACCATATATATTATGCAAATATAACT
CGTATAAACACATAAGATATAATTAAAAACGTTGGGTAGCTCCATAACGTTGATCCGAAC
ATCAAATTACCTTCCTCAAAAATAATTGATCAAAATGAAAATGCTTTTGATGACTTTTAA
CTAAATATTTTGTTTGTTAATTCAAGATGTTCTCATTAAAAGTTATTACTAATGGAGAGC
C

Last Updated on 06.12.2018 16:11