Sequences from confirmation amplicon


Confirmation sequences for line 155B08 and hit At3g02625 are shown.
Line ID 155B08
Locus At3g02625 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4138138
Primers used for PCR FA19 - 8474, show primer details
   
Sequence Name 20-K118356-0022-155-B08C-FA19
GenBank Accession LN495417 [GenBank]
Sequencing primer gene specific primer, FA19: 5'-TTTAGTCGTTTTATGAGATTGGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K118356-0022-155-B08C-FA19
CTCTATTGAATTGAAACAATGCAGGTCCTTATCCTAATTGGGCAACAATTGTTATATACA
AGTGTGAGAGTGATGATAAAGATCCACAGCTAGGATGCTATGTAATATCTAATGCAGAAA
GATAGTAATAATTCCATGGCATGCATTAAATCAGGTTGGTGGTAGCCCCTCTCTTTTGAC
CGATATTATACTATATGATAAGTTTTGTCATGAGCATGGAACATCATCTCTCTCTTCTCT
CTTGCATTGGCATTTAGTGAATGTGAATGTGAATGGAACAAGGGGACATACATATATTGG
CTGCAGAAAAAGAGAGGGCAGCTTGAGTATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
   
Sequence Name 20-K118355-0022-155-B08C-8409
GenBank Accession LN495416 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K118355-0022-155-B08C-8409
TTTACATTGAAATATACTCAAGCTGCCCTCTCTTTTTCTGCAGCCAATATATGTATGTCC
CCTTGTTCCATTCACATTCACATTCACTAAATGCCAATGCAAGAGAGAAGAGAGAGATGA
TGTTCCATGCTCATGACAAAACTTATCATATAGTATAATATCGGTCAAAAGAGAGGGGCT
ACCACCAACCTGATTTAATGCATGCCATGGAATTATTACTATCTTTCTGCATTAGATATT
ACATAGCATCCTAGCTGTGGATCTTTATCATCACTCTCACACTTGTATATAACAATTGTT
GCCCAATTAGGATAAGGACCTGCATTGTTTCAATTCATATAGAGTGGGTGGTGTTGTCAC
GAGCATTATTAATGTATAACGTAATTTCCCCCAATCTCATAAACCGACTAAA

Last Updated on 06.12.2018 16:11