Sequences from confirmation amplicon


Confirmation sequences for line 155B11 and hit At1g24380 are shown.
Line ID 155B11
Locus At1g24380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8646122
Primers used for PCR 44vd - 8474, show primer details
   
Sequence Name 31-K117776-0022-155-B11C-44vd
GenBank Accession LN500327 [GenBank]
Sequencing primer gene specific primer, 44vd: 5'-GATCAAAGAAATTCAACGGCTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K117776-0022-155-B11C-44vd
TATTGATTTGCGATATGAAATGACTAATCAATGCAACGATTAGATTCTCTTCAAAAAAAT
TCAATGGTTAAGATGAACTCATCTTTTTCTCATGAGGTGACCAGTGATAAAAGACATGAA
ACGACTAATCAGTGCAAGGGTTAGATCTTGGTAAAAAAATTCAACGGTTAAGATCAATTT
GTCTTTTTCTGAAGCTACATGGCTTCAATTGAATTGATCAATTGGTAAAGTATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTAT
   
Sequence Name 07-K117775-0022-155-B11C-8409
GenBank Accession LN489458 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K117775-0022-155-B11C-8409
ATTTACAATTGAATATACTTTACCAATTGATCAATTCAATTGAAGCCATGTAGCTTCAGA
AAAAGACAAATTGATCTTAACCGTTGAATTTTTTTACCAAGATCTAACCCTTGCACTGAT
TAGTCGTTTCATGTCTTTTATCACTGGTCACCTCATGAGAAAAAGATGAGTTCATCTTAA
CCATTGAATTTTTTTTGAAGAGAATCTAATCGTTGCATTGATTAGTCATTTCATATCGCA
AATCAATTATCACCTCATGAGAAAAAGACGAGTTCATCTTAGCCGTTGAATTTCTTTGAT
C

Last Updated on 06.12.2018 16:11