Sequences from confirmation amplicon


Confirmation sequences for line 155D01 and hit At5g16290 are shown.
Line ID 155D01
Locus At5g16290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5335088
Primers used for PCR 10et - 8474, show primer details
   
Sequence Name 50-K110926-0022-155-D01C-10et
GenBank Accession LN507204 [GenBank]
Sequencing primer gene specific primer, 10et: 5'-GATTTGCAGAGTAAGGAGGCATAC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K110926-0022-155-D01C-10et
GGTATATAATAGAATTGCTGGAGTATTTGCTAGAAGGGGATACAATATTGAGTCCCTTGC
CGTAGGATTGAATGAAGACAAGGCTCTGTTTACTATAGTTGTTCTTGGAACAGATAAAGT
CTTGCAGCAAGTTGTAGAACAGCTTAACAAGCTCGTCAATGTCATCAAGGTTTTTATCTT
GTTCTCTATTGAATCTAGTCTTGGCTATAACTTGCTGCTTTGTTTAAGAATTTTTTTGAG
CTTATATATACTTTAATGAATCATGACTATTATCTTATACTCATAATGGTTTGTTCAGGT
GGAAGATCTCTCCAAGGAGCCCCATGTAGAACGTGAGTTAATGCTCATTAAACTTAACGC
TGATCCAAGCACCCGCTCAGAGGTAAAGAATAATTCATCTTGCAAAAATATTGGGTGGAT
GTTCAAAGTTGTTTTTTCGTTATGCTTGATGCAGTTGCATTAAAAACATCAAGGATATGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCG
   
Sequence Name 34-K110925-0022-155-D01C-8409
GenBank Accession LN501457 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K110925-0022-155-D01C-8409
ATCCATATCCTTGATGTTTTTATGCAACTGCATCAAGCATAACGAAAAAACAACTTTGAA
CATCCACCCAATATTTTTGCAAGATGAATTATTCTTTACCTCTGAGCGGGTGCTTGGATC
AGCGTTAAGTTTAATGAGCATTAACTCACGTTCTACATGGGGCTCCTTGGAGAGATCTTC
CACCTGAACAAACCATTATGAGTATAAGATAATAGTCATGATTCATTAAAGTATATATAA
GCTCAAAAAAATTCTTAAACAAAGCAGCAAGTTATAGCCAAGACTAGATTCAATAGAGAA
CAAGATAAAAACCTTGATGACATTGACGAGCTTGTTAAGCTGTTCTACAACTTGCTGCAA
GACTTTATCTGTTCCAAGAACAACTATAGTAAACAGAGCCTTGTCTTCATTCAATCCTAC
GGCAAGGGACTCAATATTGTATCCCCTTCTAGCAAATACTCCAGCAATTCTATTTATTAT
ACCGCTCTCATCGCCCACAAACACCGAGATTGTATGCCTCCTTATCTTGCAAAT

Last Updated on 06.12.2018 16:11