Sequences from confirmation amplicon


Confirmation sequences for line 155H11 and hit At1g57720 are shown.
Line ID 155H11
Locus At1g57720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:21378548
Primers used for PCR 07uj - 8474, show primer details
   
Sequence Name 51-K117671-0022-155-H11C-07uj
GenBank Accession LN507623 [GenBank]
Sequencing primer gene specific primer, 07uj: 5'-CCTCAGCCTTCATTCAGATAAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K117671-0022-155-H11C-07uj
CAATACTAATCTTAGCGAGTAAGGAGTAGCATGATTTGTTAGTACAGAGAGTGCATGAGA
CAAGGCACAAAAAATGAGGGAAAGCTTACTGGGGCAGAGAAGGGAGCATAGCCCATTCTT
GGAGCAAACCACTTCAACATGTTAGCATCAATCTCCAATGAGGAGAAATCGATCCATTGC
TCAATGTGAGCCTGAAAAGTAAACACATGCACATAGACATGAATCAATGATAGTAAACAA
GAGATAAACACAAAGGTACGATAATTTTAAAAGGAAAGGCCATTGGATAAACAGGAGCTT
ACGTATTCAATAAGGGAGGATCCATTCAAAGAGTTGTCACCATTCTTACGGCTCACATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 03-K117670-0022-155-H11C-8409
GenBank Accession LN487663 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117670-0022-155-H11C-8409
CGTAGAATGGTGACACTCTTTGAATGGATCCTCCCTTATTGAATACGTAAGCTCCTGTTT
ATCCAATGGCCTTTCCTTTTAAAATTATCGTACCTTTGTGTTTATCTCTTGTTTACTATC
ATTGATTCATGTCTATGTGCATGTGTTTACTTTTCAGGCTCACATTGAGCAATGGATCGA
TTTCTCCTCATTGGAGATTGATGCTAACATGTTGAAGTGGTTTGCTCCAAGAATGGGCTA
TGCTCCCTTCTCTGCCCCAGTAAGCTTTCCCTCATTTTTTGTGCCTTGTCTCATGCACTC
TCTGTACTAACAAATCATGCTACTCCTTACTCGCTAAGATTAGTATTTGACTAGTCTAAG
CTTGACTAATCTTTTTAATGGTTTTATCTGAATAAGGGCTG

Last Updated on 06.12.2018 16:11