Sequences from confirmation amplicon


Confirmation sequences for line 156C05 and hit At5g08230 are shown.
Line ID 156C05
Locus At5g08230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:2647401
Primers used for PCR MG08 - 8474, show primer details
   
Sequence Name 76-K031454-022-156-C05C-MG08
GenBank Accession LN513325 [GenBank]
Sequencing primer gene specific primer, MG08: 5'-GTGAAAACTGTGAATGTGCTTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K031454-022-156-C05C-MG08
CATAGATCCTTCTACATGAAGAAGAAGAATCTGACATCCCAAAACTGAACAATGGGGCAT
TTCCATTTTCTTGATTGACATCAATCCTATGATAACAAAAAAAACACAAAGACTATAACC
TCCAAGCAAAATACGAACATATCAATGACATAGGAAAAGACAAAAAAAGCATACACGTCA
GCAGACAAGTTCACAAAAGTGTCCCTTGACACTTCAACATCCAGACGAGTTGCAACTTTC
GGAGTGCTAGTTGGCTTTTCTCCAAAAGCTGGTTTGCTTCTCTGGTTTGCTGTCTGAGAA
TGAGAAGAGCTTACTCTATCAGAACCAGCCACTACGTCCTTGGAAGAATCACCATGATAT
TTTTTTGGTATGCCCGCACCAGAGACTTTTGCAATTTTATTCTGTACTGCCACCAGCTCC
TTGGTTGAAAACAACTGAGGGGACTTCTTAGGAGATCTCAAGGTCTGCAATAAATGGCCA
GGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACG
ATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATT
TCGACGTGTCTACATTCA
   
Sequence Name 68-K031454-022-156-C05C-8409
GenBank Accession LN511932 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K031454-022-156-C05C-8409
TTACAATTGAATATATCCTGGCCATTTATTGCAGACCTTGAGATCTCCTAAGAAGTCCCC
TCAGTTGTTTTCAACCAAGGAGCTGGTGGCAGTACAGAATAAAATTGCAAAAGTCTCTGG
TGCGGGCATACCAAAAAAATATCATGGTGATTCTTCCAAGGACGTAGTGGCTGGTTCTGA
TAGAGTAAGCTCTTCTCATTCTCAGACAGCAAACCAGAGAAGCAAACCAGCTTTTGGAGA
AAAGCCAACTAGCACTCCGAAAGTTGCAACTCGTCTGGATGTTGAAGTGTCAAGGGACAC
TTTTGTGAACTTGTCTGCTGACGTGTATGCTTTTTTTGTCTTTTCCTATGTCATTGATAT
GTTCGTATTTTGCTTGGAGGTTATAGTCTTTGTGTTTTTTTTGTTATCATAGGATTGATG
TCAATCAAGAAAATGGAAATGCCCCATTGTTCAGTTTTGGGATGTCAGATTCTTCTTCTT
CATGTATGAAGGATCTAATTGCAGCTGCCCAGGCAAAAAGAAAACAAGCACATTCACAGT
TTTCA
Primers used for PCR 29wp - 8474, show primer details
   
Sequence Name 41-K118546w-0022-156-C05C-29wp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 29wp: 5'-AGGTGGTTACGGATCTTAACATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K118546w-0022-156-C05C-29wp
TTGAAAATGAACTAGGAAAGTCTGCATCAGGAGCAGATGAAAGTAAACGTGCTGCTAAGA
GGCCACGGTCTGAAGATGCGAAGGATCAAAAACAATGTAAGAGCAAACGTCTTGTTCCTG
TTGGTGAAGGCAAGGCTGAAATATCTGATTCTACTGGCGTTGTATCAATTTTTAAGCGTG
AGATTGTGTTAGGGATAAGTGCCCTGGGAGGAAAAAATCAATTTGATAAGGACATGGTCG
CTTATACAAAGCGCCGGAAACAGACAGTGGAGCACACAAGTGTTTCCTCTTTTCCTGGGT
CTCTCGTTAAAGAGGGGGCAAATCACCCTGAACAGAAGATAAGTTCATCTTCTGATAGCG
ATATCAAGGTTCAAGCTGCTCAGCTGCCGAAGAGACGGAGGGCGGTTTGTATCTATGACG
ACGATGATGATGATGAAGACCCAAAGACTCCAGTGCATGGAGGACTTAGCAATATTCCCA
TAGCTTCTACTGATGCTCCTAAAAGTGCTAATGCGTCCCATAATACTTCTATTAAAGCAA
AACTATTAGCTGGATCTACAGACAGTGTGAAAACAGGGAAAGTACCTCTGTATAAACATA
ACAAGGATGCTTCACTTGCACTACCTGATAGCGTGGAAGGATATAACTCACGTATGGGAA
AACCTTTCAAGGCGTTGCTCCAAAAGAACATCAAGCCGA
   
Sequence Name 21-K118545w-0022-156-C05C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118545w-0022-156-C05C-8409
TTTGATGACTGGTTTACCCATATAGGAGCTGTTTGAAGGGCCTTCCACACTATCTGGCAT
TCCTGCACCAGCGACTTTTGCAGTTTTATTTTGTCCTGTCACCTGTTTCTTAAATGAAAC
CAGCTGATAAGAATTCTTAGGAGATCTTAAGATCGGCTTGATGTTCTTTTGGAGCAACGC
CTTGAAAGGTTTTCCCATACGTGAGTTATATCCTTCCACGCTATCAGGTAGTGCAAGTGA
AGCATCCTTGTTATGTTTATACAGAGGTACTTTCCCTGTTTTCACACTGTCTGTAGATCC
AGCTAATAGTTTTGCTTTAATAGAAGTATTATGGGACGCATTAGCACTTTTAGGAGCATC
AGTAGAAGCTATGGGAATATTGCTAAGTCCTCCATGCACTGGAGTCTTTGGGTCTTCATC
ATCATCATCGTCGTCATAGATACAAACCGCCCTCCGTCTCTTCGGCAGCTGAGCAGCTTG
AACCTTGATATCGCTATCAGAAGATGAACTTATCTTCTGTTCAGGGTGATTTGCCCCCTC
TTTAACGAGAGACCCAGGAAAAGAGGAAACACTTGTGTGCTCCACTGTCTGTTTCCGGCG
CTTTGTATAAGCGACCATGTCCTTATCAAATTGATTTTTTCCTCCCAGGGCACTTATCCC
TAACACAATCTCACGCTTAAAAATTGATACAACGCCAGTAGAATCAGATATTTCAGCCTT
GCCTTCACCAACAGGGACAAGACGTTTGCTCTTACATTGTTTTTGATCCTTCGCATCTTC
AGACCGTGGGCTCTAAGCAGCACGTTTACTTTCATCTGCTCCTGATGCAGACTTTCCTAG

Last Updated on 06.12.2018 16:11