Sequences from confirmation amplicon


Confirmation sequences for line 156E10 and hit At1g18900 are shown.
Line ID 156E10
Locus At1g18900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6532322
Primers used for PCR 01cv - 8474, show primer details
   
Sequence Name 22-K109642-0022-156-E10C-01cv
GenBank Accession LN496152 [GenBank]
Sequencing primer gene specific primer, 01cv: 5'-AAAACTTGATATGGGTTTCTGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K109642-0022-156-E10C-01cv
TATGTTTCTCCTCAAGATGCCAGCTGCGGGTCCGGATGGCGAAAACGTGCGCAACCATGC
GAACAATTTCCTGGATCTTATGCATAGCGAGGACAGGGAGAGCAAGCGAGGACTCGTGGA
TGCGGTGGTTGACTTTCTACATAAGTCCGGGCAAAAAGAAGAGGCCGGATCTGTCTGGGA
AGTTGCTGCACAAAAGAATGTGTTTCCTGATGCATTAAGGGAGAAAAGCTGCAGCTACTG
GCTTATCAATCTCCATGTAATGTCAGAGGGAACTGCAGTCACCGCGCTGTCTAGGACGCT
TGCTTGGTTCCGTAAGCAGATGTTAGCTTCAGGAACTTGCCCTTCTCGGATTGATATAGT
AACTGGTTGGGGAAGACGTAGTAGAGTCACCGGTACATCAATGGTGAGACAAGCAGTTGA
GGAGCTGCTGAACATCTTTGGTTCACCGTTTTTCACGGAGAGTGGAAACTCAGGTTGTTT
CGTTGGAAGCGGAGAGCCTCTTAACAGATGGTTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCG
   
Sequence Name 06-K109633-0022-156-E10C-8409
GenBank Accession LN488855 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K109633-0022-156-E10C-8409
CATTTCACCATCTGTTAAGAGGCTCTCCGCTTCCACGAAACAACCTGAGTTTCCACTCTC
CGTGAAAAACGGTGAACCAAAGATGTTCAGCAGCTCCTCAACTGCTTGTCTCACCATTGA
TGTACCGGTGACTCTACTACGTCTTCCCCAACCAGTTACTATATCAATCCGAGAAGGGCA
AGTTCCTGAAGCTAACATCTGCTTACGGAACCAAGCAAGCGTCCTAGACAGCGCGGTGAC
TGCAGTTCCCTCTGACATTACATGGAGATTGATAAGCCAGTAGCTGCAGCTTTTCTCCCT
TAATGCATCAGGAAACACATTCTTTTGTGCAGCAACTTCCCAGACAGATCCGGCCTCTTC
TTTTTGCCCGGACTTATGTAGAAAGTCAACCACCGCATCCACGAGTCCTCGCTTGCTCTC
CCTGTCCTCGCTATGCATAAGATCCAGGAAATTGTTCGCATGGTTGCGCACGTTTTCGCC
ATCCGGACCCGCAGCTGGCATCTTGAGGAGAAACATATGTGCTGGATGACCTGTGCTTGC
CATCAACTGGCCACAGAAACCCATATCAAGTT
Primers used for PCR 13dh - 8474, show primer details
   
Sequence Name 23-K109642-0022-156-E10C-13dh
GenBank Accession LN496595 [GenBank]
Sequencing primer gene specific primer, 13dh: 5'-TGTGTAATGACTGTGGAAGAAAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K109642-0022-156-E10C-13dh
CAGTGTTCTTCTTGTGCCTCCTACAACACCAGACTTTTCTAGTTCCTTCTCCAATTTTCC
TTCTTTGTTTTCTTCTTTGTACATCTGCAAATCTTTTAACAAGTTTTTCTTTTCTTTTTG
GAAGCATTTGATTTTGTATGAAAAAAAATACTCTACATATTATTAAATCTAGATAATTGT
TTACACCTCGATCGTTTCAAGTAATCTCAAATTATTACGAGAGGAAGATACAACTGAGAA
GCTGAGGACAAAAGACTGGAGAGACACTCCATCCTGTCTAGCTCTAACCTTAACTTATGA
CTTACTAGCTAAAACAATTTCATAAGACACAATCGGCCAAAAGATAAAATAAATAAATAT
AAAAAGCCTATATTGTGATACAAAAACTCACAACCTGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 07-K109633-0022-156-E10C-oriL
GenBank Accession LN489316 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K109633-0022-156-E10C-oriL
CACAATATAGGCTTTTTATATTTATTTATTTTATCTCTTTGCCGATTGTGTCTTATGAAA
TTGTTTTAGCTAGTAAGTCATAAGTTAAGGTTAGAGCTAGACAGGATGGAGTGTCTCTCC
AGTCTTTTGTCCTCAGCTTCTCAGTTGTATCTTCCTCTCGTAATAATTTGAGATTACTTG
AAACGATCGAGGTGTAAACAATTATCTAGATTTAATAATATGTAGAGTATTTTTTTTCAT
ACAAAATCAAATGCTTCCAAAAAGAAAAGAAAAACTTGTTAAAAGATTTGCAGATGTACA
AAGAAGAAAACAAAGAAGGAAAATTGGAGAAGGAACTAGAAAAGTCTGGTGTTGTAGGAG
GCACAAGAAGAACACTTGTGATAGAGCCAATGGTAAGGAGCATTCCCCTTTCTTCCACAG
TCATTACAC
Primers used for PCR 28dj - 8474, show primer details
   
Sequence Name 20-K109674-0022-156-E10C-28dj
GenBank Accession LN495251 [GenBank]
Sequencing primer gene specific primer, 28dj: 5'-CACATATGTTTCTCCTCAAGATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K109674-0022-156-E10C-28dj
GCACCATGCGAACAATTTCCTGGATCTTATGCATAGCGAGGACAGGGAGAGCAAGCGAGG
ACTCGTGGATGCGGTGGTTGACTTTCTACATAAGTCCGGGCAAAAAGAAGAGGCCGGATC
TGTCTGGGAAGTTGCTGCACAAAAGAATGTGTTTCCTGATGCATTAAGGGAGAAAAGCTG
CAGCTACTGGCTTATCAATCTCCATGTAATGTCAGAGGGAACTGCAGTCACCGCGCTGTC
TAGGACGCTTGCTTGGTTCCGTAAGCAGATGTTAGCTTCAGGAACTTGCCCTTCTCGGAT
TGATATAGTAACTGGTTGGGGAAGACGTAGTAGAGTCACCGGTACATCAATGGTGAGACA
AGCAGTTGAGGAGCTGCTGAACATCTTTGGTTCACCGTTTTTCACGGAGAGTGGAAACTC
AGGTTGTTTCGTTGGAAGCGGAGAGCCTCTTAACAGATGGTTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCAGCGTTATTATA
   
Sequence Name 04-K109673-0022-156-E10C-8409
GenBank Accession LN487961 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K109673-0022-156-E10C-8409
TCACCATCTGTTAAGAGGCTCTCCGCTTCCAACGAAACAACCTGAGTTTCCACTCTCCGT
GAAAAACGGTGAACCAAAGATGTTCAGCAGCTCCTCAACTGCTTGTCTCACCATTGATGT
ACCGGTGACTCTACTACGTCTTCCCCAACCAGTTACTATATCAATCCGAGAAGGGCAAGT
TCCTGAAGCTAACATCTGCTTACGGAACCAAGCAAGCGTCCTAGACAGCGCGGTGACTGC
AGTTCCCTCTGACATTACATGGAGATTGATAAGCCAGTAGCTGCAGCTTTTCTCCCTTAA
TGCATCAGGAAACACATTCTTTTGTGCAGCAACTTCCCAGACAGATCCGGCCTCTTCTTT
TTGCCCGGACTTATGTAGAAAGTCAACCACCGCATCCACGAGTCCTCGCTTGCTCTCCCT
GTCCTCGCTATGCATAAGATCCAGGAAATTGTTCGCATGGTTGCGCACGTTTTCGCCATC
CGGACCCGCAGCTGGCATCTTGAGGAGAACCATATGTG

Last Updated on 06.12.2018 16:11