Sequences from confirmation amplicon


Confirmation sequences for line 156F07 and hit At2g24560 are shown.
Line ID 156F07
Locus At2g24560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10431660
Primers used for PCR 09pv - 8474, show primer details
   
Sequence Name 27-K115371-0022-156-F07C-09pv
GenBank Accession LN498526 [GenBank]
Sequencing primer gene specific primer, 09pv: 5'-GATCTCCAAGACTCCATTTTTCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K115371-0022-156-F07C-09pv
CGCATCCCAATATGATAAGTTTCACTTCCCAAGTATTTGGTCTTTTCTAGCATATATATT
GGATTTATCTAAAGAAAACTATACACTAAAAATGGTTAGCAAAAAATAACACTTTGCTTT
AAGTATTAACTTAGGTCCTCTTTTAAAACCTTGCTTAGGTAACCATATTCTCTATTCAGT
CGTTAAAATCTTTATTAATACTATAACCCGGCTTTTGTTTTTATTTTTCAGCAAAAAAAA
ACACAAACACACACACACATTTAATCAGAAATCCAGCAAGGTCCAACAAGAAGAAAAAAC
ACAATCAAAATTCCTTATAAATTAATCAAAACACTAGCTAGACAGTAGACACAATACAAA
TCAAACTAATGTCGACTTCTAAGACCATAACCTTCACTCTCTTCATAGCAGCACTACTCT
CGTCCTGCGACGCAGCCACAAACGCTACATCGCAACCACTATTTCCAGCGATTCTTATCT
TTGGCGATTCAATTGGCCATCATATTCAAGTTCGGTAATTGATGTTCTGTAGCCATATGG
GCCTTTTTTGATTTCCATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATTCC
   
Sequence Name 07-K115370-0022-156-F07C-8409
GenBank Accession LN489404 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K115370-0022-156-F07C-8409
TTCATTGAATATATCCTGATGGAAATCAAAAAAGGCCAATATGGCTACAGAACATCAATT
ACCGAACTTGAATATGATGGCCAATTGAATCGCCAAAGATAAGAATCGCTGGAAATAGTG
GTTGCGATGTAGCGTTTGTGGCTGCGTCGCAGGACGAGAGTAGTGCTGCTATGAAGAGAG
TGAAGGTTATGGTCTTAGAAGTCGACATTAGTTTGATTTGTATTGTGTCTACTGTCTAGC
TAGTGTTTTGATTAATTTATAAGGAATTTTGATTGTGTTTTTTCTTCTTGTTGGACCTTG
CTGGATTTCTGATTAAATGTGTGTGTGTGTTTGTGTTTTTTTTTGCTGAAAAATAAAAAC
AAAAGCCGGGTTATAGTATTAATAAAGATTTTAACGACTGAATAGAGAATATGGTTACCT
AAGCAAGGTTTTAAAAGAGGACCTAAGTTAATACTTAAAGCAAAGTGTTATTTTTTGCTA
ACCATTTTTAGTGTATAGTTTTCTTTAGATAAATCCAATATATATGCTAGAAAAGACCAA
ATACTTGGGAAGTGAAACTTATCATATTGGGATGCGTCTGATACAAAAACTTGGCCCCAG
AAGTTGCTGAAAAATGGAG

Last Updated on 06.12.2018 16:11