Sequences from confirmation amplicon


Confirmation sequences for line 157A09 and hit At5g48515 are shown.
Line ID 157A09
Locus At5g48515 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19659973
Primers used for PCR 20xq - 8474, show primer details
   
Sequence Name 50-K119512-0022-157-A09C-20xq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 20xq: 5'-ATTAATCATCTCAAAGGTACCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K119512-0022-157-A09C-20xq
TGTTGTTGATTGTTTCGTGATTCCTAATGAGAGAATTGGTGATTGATATTGATCATACAG
TTATTCTACTATGAATGATGAAGGTTGTGAATGATGAAGAATCTCTCTGAAGGACAAATT
CGAAAACGAGTTACTCAACATATTGAAAGTTCTCTTAACGGCCTCACACTTTAAGATATT
TCCTTTTTAAGTATGATATCTCGGTTTGTAGTGAATTCAATATTTTTTGTGGTTTTTGAA
CCCAATTCCTGCTATGGAGATAAATGTAGGCTACTCTGACTCCAACGATTAATCCATATA
GTTCCTACTAGCAAATCTGTCTAGTATATGAATTCTATCTCAATACCACTCGATCTCTAG
AATCCATTCACTGCTTTAACTTAGGGCCATAATGCAAGCTTAATCTCACTTACCCCGCGG
CAGAGCTTATATGACAAAAGCAGACAATCACTAGGTGAAAACAAGTCACAACCAAATTCG
TTGAGGGACCAATATTCAGAGCATGACACAATCTTCTTCAACTTCTTCATTTTCTCTCGG
CAAGATCAAGTCAACTTGAACTCAAAGGCCAAACTTAGATTATTTATATTAGAATGACAT
ATAAAACATGGAAAAAAAATATAGTAAATTATTATAAAATAATGTCTTACAAAAAAATAA
TGTCTTACAAAAAAAATAATGTCTTACAAAATGTTTTCCAATGTACAACGACNAATAGAA
TTTTCACAATGAGGTATATTCTTCTTTTTCTCAATACAATGAAATGCACATTGTTGGCAA
AAGATAATTTTCATGTCATCAGGATATATTCAAATTGTAATGGCTTCATGTCCGGGAAAT
CTACATGGATCAGCAATGAGTATGATGTTCAATATGGAGAAAA
   
Sequence Name 18-K119511-0022-157-A09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K119511-0022-157-A09C-8409
CATTTCATTGAATATATCCTGATGACTGAAAATTATCTTTTGCCAACAATGTGCATTTCA
TTGTATTGAGAAAAAGAAGAATATACCTCATTGTGAAAATTCTATTTGTCGTTGTACATT
GGAAAACATTTTGTAAGACATTATTTTTTTTGTAAGACATTATTTTTTTGTAAGACATTA
TTTTATAATAATTTACTATATTTTTTTTCCATGTTTTATATGTCATTCTAATATAAATAA
TCTAAGTTTGGCCTTTGAGTTCAAGTTGACTTGATCTTGCCGAGAGAAAATGAAGAAGTT
GAAGAAGATTGTGTCATGCTCTGAATATTGGTCCCTCAACGAATTTGGTTGTGACTTGTT
TTCACCTAGTGATTGTCTGCTTTTGTCATATAAGCTCTGCCGCGGGGTAAGTGAGATTAA
GCTTGCATTATGGCCCTAAGTTAAAGCAGTGAATGGATTCTAGAGATCGAGTGGTATTGA
GATAGAATTCATATACTAGACAGATTTGCTAGTAGGAACTATATGGATTAATCGTTGGAG
TCAGAGTAGCCTACATTTATCTCCATAGCAGGAATTGGGTTCAAAAACCACAAAAAATAT
TGAATTCACTACAAACCGAGATATCATACTTAAAAAGGAAATATCTTAAAGTGTGAGGCC
GTTAAGAGAACTTTCAATATGTTGAGTAACTCGTTTTCGAATTTGTCCTTCAGAGAGATT
CTTCATCATTCACAACCTTCATCATTCATAGTAGAATAACTGTATGATCAATATCAATCA
CCAATTCTCTCATTAGGAATCACGAAACAATCAACAACACATTCCACTTTAATAAAGGCT
TGCTAGTCCAAGTGCGCG

Last Updated on 06.12.2018 16:11