Sequences from confirmation amplicon


Confirmation sequences for line 157C09 and hit At1g11220 are shown.
Line ID 157C09
Locus At1g11220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3760504
Primers used for PCR 16ms - 8474, show primer details
   
Sequence Name 90-K114351-0022-157-C09C-16ms
GenBank Accession LN515057 [GenBank]
Sequencing primer gene specific primer, 16ms: 5'-TTCTACTCTTCGATTCAGCTCCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K114351-0022-157-C09C-16ms
CGATAATTAGTCACGNTCCCTGNNAACGTCTCCGCTTTCCTCAAGACAGGGTTTTACATC
GCCGCCGGCGNTTAAGTCCAGACATACTGGGTTTTCCGGTAATGACAAGTCAACGGCNGT
TGATTTTCCCTCCTCAGTTATCATGTTCCACGTGTTCTCCAGTGTCTCGTGCCGGTTCGG
TTTCACCACTTTCAACGCCTTCTTTTTTCTATTAACGCCTGCAAAATAAGAATTTCCTAT
ATAAGACAATTACCTATTTACCCCTCTACGGTTTATTTCGGTTTAAACCGGTTATAATCG
GTCATTGTCGTCGATATAGCGTGAAAATTCAAATAAAATCTAGCCGTTATGTTGAATGAC
GTCATCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
   
Sequence Name 42-K114350-0022-157-C09C-8409
GenBank Accession LN504550 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K114350-0022-157-C09C-8409
CCGGAGAGTCTTCACAAACGGCTAGATTTTATTTGAATTTTCACGCTATATCGACGACAA
TGACCGATTATAACCGGTTTAAACCGAAATAAACCGTAGAGGGGTAAATAGGTAATTGTC
TTATATAGGAAATTCTTATTTTGCAGGCGTTAATAGAAAAAAGAAGGCGTTGAAAGTGGT
GAAACCGAACCGGCACGAGACACTGGAGAACACGTGGAACATGATAACTGAGGAGGGAAA
ATCAACGCCGTTGACTTGTCATTACCGGAAAACCAGTATGTCTGGACTTAACGCCGGCGG
CGATGTAAAACCTGTCTTGAGGAAAGCGGAGACGTTCAGGGACGTGACTAATTATCGACA
GTCGTCTCCGACGGTGACGTCACCGGTGAAGATGAAGAAGGAGATGTCGCCGAGTAGGGA
GGAGCTG

Last Updated on 06.12.2018 16:11