Sequences from confirmation amplicon


Confirmation sequences for line 157D10 and hit At3g22200 are shown.
Line ID 157D10
Locus At3g22200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:7837002
Primers used for PCR RK63 - 8474, show primer details
   
Sequence Name 24-K020068-022-157-D10C-8409
GenBank Accession LN496813 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K020068-022-157-D10C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATAGCCATTTACATGGGGAA
GGCTGGAACAACAAACTAACTTTGTTAGGGAAAAGAAGGAATCTTTAGCACTTCTAGAAA
GAACATATGAGGAACAATATTTATCCTACCCGGACAAACTTGCTGATATTAGAGTGGAGC
CATGGTACCTGAAATGATAACATTGCATTTGTAAACACTCTGAGATCTTATAAAAACAGA
CAAATGGAAAAGGACAAGCAAAAATCATTACTCACGATTTCTTTCTCGCGATAAACTTTT
TCTTCTCGGGCCTTCCAAGTGCGTTATTGTAATACCAAACCAGCTTGACCTGCATTATAT
ATACAGGAAATTCATAAACAACTCTTATAGGTCGAAAACTTAGAAAATACGAGATCAAGG
TTTGAAGTATAGTTATGGAAACCTGGGTATCGTTGGCATCTGATCCACCGCTTGTAAAAA
ATGCTTTGGCCATTTTGTTGCCGTGAACATCTCTAAAAGAACCTTAGCAAGATCCTGTTT
AGTATGAAAACATCACATTGCTCAAATTCTCTTTGATATCTCATAAAGTATACTGCTCTG
AGTCTTAACAGGACACAGAGAGTAGCAGGAAATTGTAGGAAAACATTATAAAAATGA
   
Sequence Name 23-K020068-022-157-D10C-RK63
GenBank Accession LN496372 [GenBank]
Sequencing primer gene specific primer, RK63: 5'-CTTTCCCTTTTGGTGTCATTTTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K020068-022-157-D10C-RK63
TTTTCCTACCATTTCCTGCTACTCTCTGTGTCCTGTTAAGACTCAGAGCAGTATACTTTA
TGAGATATCAAAGAGAATTTGAGCAATGTGATGTTTTCATACTAAACAGGATCTTGCTAA
GGTTCTTTTAGAGATGTTCACGGCCAACAAAATGGCCAAAGCATTTTTTACAAGCGGTGG
ATCAGATGCCAACGATACCCAGGTTTCCATAACTATACTTCAAACCTTGATCTCGTATTT
TCTAAGTTTTCGACCTATAAGAGTTGTTTATGAATTTCCTGTATATATAATGCAGGTCAA
GCTGGTTTGGTATTACAATAACGCACTTGGAAGGCCCGAGAAGAAAAAGTTTATCGCGAG
AAAGAAATCGTGAGTAATGATTTTTGCTTGTCCTTTTCCATTTGTCTGTTTTTATAAGAT
CTCAGAGTGTTTACAAATGCAATGTTATCATTTCAGGTACCATGGCTCCACTCTAATATC
AGCAAGTTTGTCCGGGTAGGATAATATTGTCTCTATGTTCTTTCTAGAAGTGCTAAAGAT
TCCTTCTTTTCCCTAACAAAGTTAGTTTGTTGTTCCAGCCTTCCCCATGTAAATGGCTAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA

Last Updated on 06.12.2018 16:11