Sequences from confirmation amplicon


Confirmation sequences for line 157H01 and hit At5g43750 are shown.
Line ID 157H01
Locus At5g43750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17580861
Primers used for PCR 08cc - 8474, show primer details
   
Sequence Name 72-K117751-0022-157-H01C-08cc
GenBank Accession LN512795 [GenBank]
Sequencing primer gene specific primer, 08cc: 5'-TTACGAACTTGTCAGTCACTTTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K117751-0022-157-H01C-08cc
ATCTCTTTCGCTAACGAACACACAAAAAAAAAAAAACCCTTTTTTCCTTCCTTGGGCCCC
AAACCTCTGAACCGGACCAGAAAAAAAATAAGGGGGTTGAAAAATGGGCACCGGCCCCAT
TTCTCCCCCCCAATCCATTCCCAAGGGCCCCGGTTCCCAATTCCGAACCTGGGTTTCCGA
ACCAAACCGCCATGGCCTAAAAAGGCGCCAATCCCGCCCGAAAATTAAATTTACCAAACC
GGGCCCCACCGCCGGGTTTGCCCCAATTCAACCCAAACTCCACCAAAACCCCATTGGGCC
ATTTCAAACCAAAAACCTTTGAATGGGAAAAAATCCATTGGTTTTAAATTCCAAATTTCG
CCGAAAATTTTTTCCGAATGGTTAACCCTTTTTTTAACCGCCATCCTCCTGGGTTCCCCA
TGGATTTTAAGGGCCAATTGGGAAAAAAAAAAAAATTATTTCCCATTTTTTTTCCATTCC
AAAATGGTA
   
Sequence Name 47-K117750-0022-157-H01C-8409
GenBank Accession LN506322 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K117750-0022-157-H01C-8409
GCGCTAACAATCAGAAACAACTTTTCAGCAGAATTATGAATTTAAAATCCAATTGAATCT
TTACCATTTCAATGCTATTAGTCTCGAATTGACCAATCGGGTCTTCGTTGATGTCTGGTT
CGATCTGTGACAATCCAGCCGCTGAGACCAGCTTCGCTAACTTCAATCTCCGGCCGGATT
TGCCGCATTTTACGACATTGACGATCTGATCAGAAACCCTAGCTCCGAATTTGGAATCAG
TGACCTTTGGAATCGATTTGGGTGAGAGAATCGTGACCGTTGCCATTTTTTCACTCACTT
TATCTTTTTCTTGCTCTGTCTCAGAGTTTTGGAGCCATAGAAAGAAAAAACGGTTCTTTT
TTTTTTGGGGGGTCCTTTACCAAAAAAAATAATAATTTTCCGGTTACCGGCCGGCCAATT
ACCCAA

Last Updated on 06.12.2018 16:11