Sequences from confirmation amplicon


Confirmation sequences for line 158F08 and hit At2g24020 are shown.
Line ID 158F08
Locus At2g24020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10218612
Primers used for PCR AH05 - 8474, show primer details
   
Sequence Name 78-K105358-0022-158-F08C-AH05
GenBank Accession LN513691 [GenBank]
Sequencing primer gene specific primer, AH05: 5'-ACCAATTTCACCAAATCTATGCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K105358-0022-158-F08C-AH05
TCTAATCCTACTTCATCTCTCGCTTAGGTTTCGTGACACTGGATTTTGAGTCTTTATGTA
TCTGTCGTACATTAGGTTTCATCTGAATTTGCTTAATCTTTCGTAATTAGACTTTTCTAT
TCCATAATTGGAGGAATTGAAGCGTAATTTTGTATTTATAAGTGTTTTTGGGATTGAAAT
GGGCTTTAGTAATTTTTTGGTTTGAATAGTTTCAGGAAATGCGAGCTTGAATTCGCAGAG
AAGAACATGGCCAAAGCAGTACAAGTCTAAAAATGGTTACAGGTCTCTTCGAGTGAATGG
ATTATTTGGAGGTGGAAACAAAGATAATAACAGTGAAGATGGACAATCAAAGGTTGGTAC
ATTACATTGGTTTTTTCAAATATCAAACAGATTCTGCAATAGGTGAACATTTTAAGTTAG
GCTTCATGTTGTAAAGATTCAAAGTTTATGATTTTGGAACTAATGAAGTTTGTGTGTGAT
CTTTCCTAGGCAGGAATATTTGGTAATATGCAGAATATGTATGAGACTGTGAAGAAAGCT
CANATGGTTGTTCAAGTGGAAGCTGTACGTGTTCAAAAAGAGTATGATGGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 62-K105359-0022-158-F08C-8409
GenBank Accession LN510757 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K105359-0022-158-F08C-8409
TTTACCATATACTCTTTTTGACACGTACAGCTTCCACTTGAACAACCATTTGAGCTTTCT
TCACAGTCTCATACATATTCTGCATATTACCAAATATTCCTGCCTAGGAAAGATCACACA
CAAACTTCATTAGTTCCAAAATCATAAACTTTGAATCTTTACAACATGAAGCCTAACTTA
AAATGTTCACCTATTGCAGAATCTGTTTGATATTTGAAAAAACCAATGTAATGTACCAAC
CTTTGATTGTCCATCTTCACTGTTATTATCTTTGTTTCCACCTCCAAATAATCCATTCAC
TCGAAGAGACCTGTAACCATTTTTAGACTTGTACTGCTTTGGCCATGTTCTTCTCTGCGA
ATTCAAGCTCGCATTTCCTGAAACTATTCAAACCAAAAAATTACTAAAGCCCATTTCAAT
CCCAAAAACACTTATAAATACAAAATTACGCTTCAATTCCTCCAATTATGGAATAGAAAA
GTCTAATTACGAAAGATTAAGCAAATTCAGATGAAACCTAATGTACGACAGATACATAAA
GACTCAAAATCCAGTGTCACGAAACCTTAAGCGAGAGATAGAAGTAGGGATTTAGGAGAG
ACTAACCATGAGAGAATGGAAAGAGCATAGATTTGGTGAAAATTGG

Last Updated on 06.12.2018 16:11