Sequences from confirmation amplicon


Confirmation sequences for line 158H04 and hit At1g22880 are shown.
Line ID 158H04
Locus At1g22880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8095865
Primers used for PCR MK77 - 8474, show primer details
   
Sequence Name 88-K101329-0022-158-H04C-8409
GenBank Accession LN514841 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K101329-0022-158-H04C-8409
CTTTACATTGAATATATCCTGACTTTGAGAGTGCTTCTCTGTAATTGGGACTTGCATAGG
TATTCTCCAGTGAAAGCGCAGAGAGAAGGAACACAAAGAAGAAAGGAGAAGCCATTCTTG
TTATTGTGTTGGCTTGAGGGTACGACTTTATCACACACGTATATATATAGCTAGAGAAGG
AGACCGGGGCTGATGAGATTCAGAGCGAAGATTATGGCTTTTTGAAATTCAGCTTAACTG
CTAAAAAAACTGTTCTTAGTTACTTCAATTTGAATTTCCTTTTTAATTAATATTTTATAG
AACTGACAAGTGGAGGATTACATGTATTATTCTGTAATCTTCTTAATATGTTCAATTTTT
TTTTAACTAATAGTTTGATTACCACATAGGTTTGTCTATATGAGTTCACCGACACAAATT
GGTAATTTCTTGGTAGTTTTGATGTTTGATACTTATCTNTTTTACCACAGTAAACTGTAA
TGAAGTGTATTTTAGATAGTGGCATTGCGTAATACGTACGATTAGGGGATAGATAAACAA
TGTCTTAAATCTTGATAATAGGGTAGTTAGTTAGGTAGTAATTAGTTTAGTTAGGTTAGG
GATTTTGG
   
Sequence Name 88-K101335-0022-158-H04C-MK77
GenBank Accession LN514842 [GenBank]
Sequencing primer gene specific primer, MK77: 5'-ATCATAATCCCCAAAATCCCTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K101335-0022-158-H04C-MK77
CCCTATTATCAAGATTTAAGACATTGTTTATCTATCCCCTAATCGTACGTATTACGCAAT
GCCACTATCTAAAATACACTTCATTACAGTTTACTGTGGTAAAAAAGATAAGTATCAAAC
ATCAAAACTACCAAGAAATTACCAATTTGTGTCGGTGAACTCATATAGACAAACCTATGT
GGTAATCAAACTATTAGTTAAAAAAAAATTGAACATATTAAGAAGATTACAGAATAATAC
ATGTAATCCTCCACTTGTCAGTTCTATAAAATATTAATTAAAAAGGAAATTCAAATTGAA
GTAACTAAGAACAGTTTTTTTAGCAGTTAAGCTGAATTTCAAAAAGCCATAATCTTCGCT
CTGAATCTCATCAGCCCCGGTCTCCTTCTCTAGCTATATATATACGTGTGTGATAAAGTC
GTACCCTCAAGCCAACACAATAACAAGAATGGCTTCTCCTTTCTTCTTTGTGTTCCTTCT
CTCTGCGCTNTCACTGGAGAATACCTATGCAAGTCCCAATTACAGAGAAGCACTCTCAAA
GTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCCATTCAN
AAATGT

Last Updated on 06.12.2018 16:11