Sequences from confirmation amplicon


Confirmation sequences for line 159C08 and hit At2g19650 are shown.
Line ID 159C08
Locus At2g19650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8494257
Primers used for PCR 11pv - 8474, show primer details
   
Sequence Name 33-K115331-0022-159-C08C-11pv
GenBank Accession LN501120 [GenBank]
Sequencing primer gene specific primer, 11pv: 5'-CAAAAGTATAGATCAGCTCCGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K115331-0022-159-C08C-11pv
ACGCAGCAAAGTGGTCTACAATAGAGTGGTTGGATACAAATGTTCCTATCCATGTTAACG
CTCCTGGCATGGAATATGCGTAAGTATCTATAACGCAGGAAATATGCAATGTGATCCCAC
ACTCATTGCATGTATAGAACCATTTTTCTGGGTTCACAGTTGTTTCGCATGCTTCACACC
AATATTTGCCAGCCACGCCCATTTCGCCATAAGATAAGAAGAGAGGATGATCGTCGTACC
TATGCCTCATTACCTTTTGCGGTAAAATAGCACACTTGAAGCACAAACCAAATTCACAAT
CATCGCAACTCAATTTCCCGTCTCCATCACACTCACTACAACGTTTGGAGTGATCTGTAG
AGTAGTGTAAAGGATGTTGATGGCTTTTGTGCTCAAATGGTTCTGTAACTGTAGCGCACC
GTACATCTATCCTTAATTCCTTAGATTCATAGGAGAAACCGGTGAACAGTTGACGGCAAA
TACTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCC
GCA
   
Sequence Name 09-K115330-0022-159-C08C-8409
GenBank Accession LN490316 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K115330-0022-159-C08C-8409
AGTATTTGCCGTCAACTGGTTCACCGGTTTCTCCTATGAATCTAAGGAATTAAGGATAGA
TGTACGGTGCGCTACAGTTACAGAACCATTTGAGCACAAAAGCCATCAACATCCTTTACA
CTACTCTACAGATCACTCCAAACGTTGTAGTGAGTGTGATGGAGACGGGAAATTGAGTTG
CGATGATTGTGAATTTGGTTTGTGCTTCAAGTGTGCTATTTTACCGCAAAAGGTAATGAG
GCATAGGTACGACGATCATCCTCTCTTCTTATCTTATGGCGAAATGGGCGTGGCTGGCAA
ATATTGGTGTGAAGCATGCGAAACAACTGTGAACCCAGAAAAATGGTTCTATACATGCAA
TGAGTGTGGGATCACATTGCATATTTCCTGCGTTATAGATACTTACGCATATTCCATGCC
AGGAGCGTTAACATGGATAGGAACATTTGTATCCAACCACTCTATTTGTAGACCACTTTG
CTGCGTGTGCAACTCTCGATGCAAGCTCCCTTTCGTTCGTACGCTTCCCGAGGACGGAGC
TGATCTATACTTTTG

Last Updated on 06.12.2018 16:11