Sequences from confirmation amplicon


Confirmation sequences for line 160E10 and hit At1g48640 are shown.
Line ID 160E10
Locus At1g48640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:17988834
Primers used for PCR 12st - 8474, show primer details
   
Sequence Name 66-K116641-0022-160-E10C-12st
GenBank Accession LN511691 [GenBank]
Sequencing primer gene specific primer, 12st: 5'-CATTGGGATAATGATACCGTTCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K116641-0022-160-E10C-12st
GTTTGCTCCACATCGTATTTNTGTAAGTGTATATATTGGTTCTTAAATTTTGAACTTAAC
GCAGATTAATTTTCATGTATAGTAAATTTCAAATAGTGTATATATAAACTTTGTATGCTT
TACAATTTGTTATGCAGCTTCCTTGCATAATGTGGCTGCTAATCTATAAACCAAAGAGGT
TTAGCCTGTCGTGGTGGACGAATTGGGTAATATCTCTTTTTTTGGGTCCAAATTCGTTAA
TTCTTTAGAATTACATTATTTCAAATTAGTGCTTAACTTTCAAAACCGGCCTTGGACTTT
AGAACCGGTGGCTAATGAATATGATGGATCAATTGTAAATTATAAAAAATTGATCAATTT
TTATTTACATGAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCG
   
Sequence Name 53-K116640-0022-160-E10C-8409
GenBank Accession LN508272 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K116640-0022-160-E10C-8409
ATCCTCATGTAAAAAAAATTGATCAATTTTTTATAATTTACAATTGATCCATCATATTCA
TTAGCCACCGNGTTCTAAAGTCCAAGGCCGNGTTTTGAAAGTTAAGCACTAATTTGAAAT
AATGTAATTCTAAAGAATTAACGAATTTGGACCCAAAAAAAGAGATATTACCCAATTCGT
CCACCACGACAGGCTAAACCTCTTTGGTTTATAGATTAGCAGCCACATTATGCAAGGAAG
CTGCATAACAAATTGTAAAGCATACAAAGTTTATATATACACTATTTGAAATTTACTATA
CATGAAAATTAATCTGCGTTAAGTTCAAAATTTAAGAACCAATATATACACTTACAAAAT
ACGATGTTGGAGCAAACGCAAATCCTCCAAAGAAAGCGAGAAGGCCCCCAAAGAACGGTA
TCTTTGTCCCAATG

Last Updated on 06.12.2018 16:11