Sequences from confirmation amplicon


Confirmation sequences for line 161H11 and hit At1g32250 are shown.
Line ID 161H11
Locus At1g32250 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11640301
Primers used for PCR BL43 - 8474, show primer details
   
Sequence Name 23-K105062-0022-161-H11C-BL43
GenBank Accession LN496549 [GenBank]
Sequencing primer gene specific primer, BL43: 5'-AGACCAATTCGAAACGCTAATAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K105062-0022-161-H11C-BL43
CCGGAGTTCGTAGCTTTGGTCTCTCCGGAGCTTCTTTCGCCGGCGAAGAGGACAACTCCT
TACACGGAGGAGCAGCTTCTCCGATTGTTTAGGATATTCGACACGGATGGCAACGGGTTT
ATCACTGCGGCGGAATTGGCTCACTCTATGGCTAAGCTTGGCCACGCCTTGACGGTGGCG
GAATTGACGGGGATGATAAAGGAGGCGGATAGCGATGGCGATGGCCGGATTAACTTCCAG
GAGTTCGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTTATT
   
Sequence Name 07-K105063-0022-161-H11C-8409
GenBank Accession LN489263 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K105063-0022-161-H11C-8409
ATTTACATTGAATATATCCTGCGAACTCCTGGAAGTTAATCCGGCCATCGCCATCGCTAT
CCGCCTCCTTTATCATCCCCGTCAATTCCGCCACCGTCAAGGCGTGGCCAAGCTTAGCCA
TAGAGTGAGCCAATTCCGCCGCAGTGATAAACCCGTTGCCATCCGTGTCGAATATCCTAA
ACAATCGGAGAAGCTGCTCCTCCGTGTAAGGAGTTGTCCTCTTCGCCGGCGAAAGAAGCT
CCGGAGAGACCAAAGCTACGAACTCCGGGAACTCTACGAGTCCGTTGCTTTTAGTGTCGG
CTTTGTCTATTAGCGTTTCGAATTGGT

Last Updated on 06.12.2018 16:11