Sequences from confirmation amplicon


Confirmation sequences for line 164D09 and hit At5g52450 are shown.
Line ID 164D09
Locus At5g52450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21291052
Primers used for PCR BM06 - 8474, show primer details
   
Sequence Name 17-K015834-022-164-D09C-8409
GenBank Accession LN493649 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K015834-022-164-D09C-8409
TAGATTTCCCGGACATTTTTGCCATTTTATGGACAGATCGAGAAGTGAAAACGCCAAAAT
AACAAAATCAAAATGTTTTTCTTGTATAAACAAAAATGAATGCTTTAAGCAGTTGCTTAT
GGCTCTTATTTGAACTTGAAAACCACTTGTCGAACATTGAACGATGTCAACAAATCCAAA
TTTTAATATTCAATTGAAAGTTTTAAAAATTGGGTTGTACGTTTTTACTTGTGAAGCAAG
AAACTACACTGATTCACTTTTCCATTTTCCTCATGAACAGATGGGAACAGCAAGTGCGTT
AGATACGCTTTGTGGCCAAGCGTACGGAGCAAAGAAGTACGGAATGTTAGGAATACAAAT
GCAAAGAGCTATGTTTGTTCTTACACTAGCTTCTATTCCTCTCTCCATCATTTGGGCTAA
CACAGAGCACCTTCTCGTCTTCTTTGGACAAAACAAATCCATTGCTACACTCGCTGGCTC
CTACGCAAAATTCATGATCCCAAGCATCTTCGCCTACGGTCTTCTTCAATG
   
Sequence Name 18-K015834-022-164-D09C-BM06
GenBank Accession LN494090 [GenBank]
Sequencing primer gene specific primer, BM06: 5'-CCTGTTAAAGCATTGAAGAAGACC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K015834-022-164-D09C-bm06
AGCGAAGATGCTTGGGATCATGAATTTTGCGTAGGAGCCAGCGAGTGTAGCAATGGATTT
GTTTTGTCCAAAGAAGACGAGAAGGTGCTCTGTGTTAGCCCAAATGATGGAGAGAGGAAT
AGAAGCTAGTGTAAGAACAAACATAGCTCTTTGCATTTGTATTCCTAACATTCCGTACTT
CTTTGCTCCGTACGCTTGGCCACAAAGCGTATCTAACGCACTTGCTGTTCCCATCTGTTC
ATGAGGAAAATGGAAAAGTGAATCAGTGTAGTTTCTTGCTTCACAAGTAAAAACGTACAA
CCCAATTTTTAAAACTTTCAATTGAATATTAAAATTTGGATTTGTTGACATCGTTCAATG
TTCGACAAGTGGTTTTCAAGTTCAAATAAGAGCCATAAGCAACTGCTTAAAGCATTCATT
TTTGTTTATACAAGAAAAACATTTTGATTTTGTTATTTTGGCGTTTTCACTTCTCGATCT
GTCCATAAAATGGCAAAAATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA

Last Updated on 06.12.2018 16:11