Sequences from confirmation amplicon


Confirmation sequences for line 164D11 and hit At1g02520 are shown.
Line ID 164D11
Locus At1g02520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:525150
Primers used for PCR 82yp - 8474, show primer details
   
Sequence Name 19-K121793-0022-164-D11C-82yp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 82yp: 5'-TACTTACATTGAACCCGCAACTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K121793-0022-164-D11C-82yp
CGCCCCGGTTACCTTTTTCAGTACATCTTCCCACAAACCAAATAGCCAAAGCATAGCTGC
TGAAAAATACAAAGAACATTACTCCAAGTCCTAAACCGGTGGAGAAACCTTGTTGAATGC
TTGATTTATACGCCGATGTTATGAACTTCTTGTAGCTGTTAATGGCTTGCTTCTCTCCAG
TAAAAGAAGCAACCTTCAAACCAAAACAAAACAAAAAGTCAAGAAGCAACTGTCAGTTAA
AGATTGTTTTGAAACTTATAGAAATGTATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAGATTATTATAAT
   
Sequence Name 03-K121792-0022-164-D11C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K121792-0022-164-D11C-8409
TTACATTGAAATACATTTCTATAAGTTTCAAAACAATCTTTAACTGACAGTTGCTTCTTG
ACTTTTTGTTTTGTTTTGGTTTGAAGGTTGCTTCTTTTACTGGAGAGAAGCAAGCCATTA
ACAGCTACAAGAAGTTCATAACATCGGCGTATAAATCAAGCATTCAACAAGGTTTCTCCA
CCGGTTTAGGACTTGGAGTAATGTTCTTTGTATTTTTCAGCAGCTATGCTTTGGCTATTT
GGTTTGGTGGGAAGATGATACTCGAAAAAGGTTATACCGGTGGCGCTGTGATCAACGTGA
TCATCATTGTAGTTGCGGGTTCAATGTAAGT

Last Updated on 06.12.2018 16:11