Sequences from confirmation amplicon


Confirmation sequences for line 164D12 and hit At4g00940 are shown.
Line ID 164D12
Locus At4g00940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:403581
Primers used for PCR IW06 - 8474, show primer details
   
Sequence Name 58-K016442-022-164-D12C-8409
GenBank Accession LN509565 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K016442-022-164-D12C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTATCAAGAGTATGA
TCATGAGCACCCCAAAAAGAATCTTGGGTTTTCATTAGACAGGAACGAGGAAGAGATTGG
TAATCATGATAACTTCGTTGTTAATGAGGAAGGAAGTAAGATGATGTATCCTTATGGAGA
TCATGAAGACCGTCAACAACATCACCATGTGAGACACGATGATGGTAATAAGAAGAGAGA
AGGTGGTTCAAGCAATGAGCTATGGAGCGGAATCATCCTAGGTGGTGATAGTGGTGGACC
AACATGGTGATATGAATATTGTCAAAAAGGACAAGATGATAAAGACAAAAACAAATAGTT
ACGAGCAAAAATATGTGATTATATTTGGAATTTTCAGACCCGGAAGAGTCGCCTTGGGAG
GAGCATGATGATTCTTCTTTCCACTTTCTATAAATTCCTCTGTTCACTTTTCTTTGTCTT
TTGTC
   
Sequence Name 62-K016442-022-164-D12C-IW06
GenBank Accession LN510638 [GenBank]
Sequencing primer gene specific primer, IW06: 5'-GGCTCCATTAGACAAAAGACAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K016442-022-164-D12C-iw06
GAACAGAGAATTTATAGAAAGTGGAAAGAAGAATCATCATGCTCCTCCCAAGGCGACTCT
TCCGGGTCTGAAAATTCCAAATATAATCACATATTTTTGCTCGTAACTATTTGTTTTTGT
CTTTATCATCTTGTCCTTTTTGACAATATTCATATCACCATGTTGGTCCACCACTATCAC
CACCTAGGATGATTCCGCTCCATAGCTCATTGCTTGAACCACCTTCTCTCTTCTTATTAC
CATCATCGTGTCTCACATGGTGATGTTGTTGACGGTCTTCATGATCTCCATAAGGATACA
TCATCTTACTTCCTTCCTCATTAACAACGAAGTTATCATGATTACCAATCTCTTCCTCGT
TCCTGTCTAATGAAAACCCAAGATTCTTTTTGGGGTGCTCATGATCATACTCTTGATAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGAT
CCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTC
GACGTGTCTACATT

Last Updated on 06.12.2018 16:11