Sequences from confirmation amplicon


Confirmation sequences for line 164D12 and hit At5g20490 are shown.
Line ID 164D12
Locus At5g20490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6930691
Primers used for PCR 27nh - 8474, show primer details
   
Sequence Name 30-K114649-0022-164-D12C-27nh
GenBank Accession LN499831 [GenBank]
Sequencing primer gene specific primer, 27nh: 5'-AATATGGCCTTTTTCTGTTGATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K114649-0022-164-D12C-27nh
GCTGATCTGCTTTTCTTGTAGCATTTTCAAGCTCTGTCGCCAGCTCGGAATTTCTAGCTT
CTGCCTCAGAGAAAGCTTTTCTCAAATTTTCTGCGGCTTGTCGTTCAGCCTGAAGCGAAG
CCTGTAATAAAAGATGTATTAAACCAACCAATCCACTTCACGTTATTAAGAGACTAATGA
ACATATATCTACTAACTTTGTTAGTATACATAAAGGATGGTCTAATACGTGAAAACACAT
ACCTTTAGAGCTTCCACCTCTGATGTTAATGAATTGATTTTTTCAGTATCCTCAACCAAT
ACGGGAGTCTCCTTAATGACTGGAGGTGCTTCTTCAATAGCTTTCCTTGCAGCCTCCCGC
TCTCTAATTACAGCAGCGTTTGCTTCTTCAACTTGCAACCGCATTGTCTCTAAAGCCTCC
TGTTGTTTTGCGTATTCTTGGGTTTTTGCTTCTTCCAGCTCGGTCTATATNAGCAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TTC
   
Sequence Name 01-K114648-0022-164-D12C-8409
GenBank Accession LN486679 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K114648-0022-164-D12C-8409
TCATTGAATATATCCTGCTAATATAGACCGAGCTGGAAGAACACAAACCCAAGAATACGC
AAAACAACAGGAGGCTTTAGAGACAATGCGGTTGCAAGTTGAAGAAGCAAACGCTGCTGT
AATTAGAGAGCGGGAGGCTGCAAGGAAAGCTATTGAAGAAGCACCTCCAGTCATTAAGGA
GACTCCCGTATTGGTTGAGGATACTGAAAAAATCAATTCATTAACATCAGAGGTGGAAGC
TCTAAAGGTATGTGTTTTCACGTATTAGACCATCCTTTATGTATACTAACAAAGTTAGTA
GATATATGTTCATTAGTCTCTTAATAACGTGAAGTGGATTGGTTGGTTTAATACATCTTT
TATTACAGGCTTCGCTTCAGGCTGAACGACAAGCCGCAGAAAATTTGAGAAAAGCTTTCT
CTGAGGCAGAAGCTAGAAATTCCGAGCTGGCGACAGAGCTTGAAAATGCTACAAGAAAAG
CAGATCAGCTTCATGAATCAGTACAAAGGTTCGTAAAATATATTGATGTACAATCAACAG
AAAAGGG

Last Updated on 06.12.2018 16:11